Modulation of L-type $Ca^{2+}$ Channel Currents by Various Protein Kinase Activators and Inhibitors in Rat Clonal Pituitary $GH_3$ Cell Line

  • Bae, Young-Min (Departments of Physiology and National Research Laboratory for Cellular Signalling, Seoul National University College of Medicine) ;
  • Baek, Hye-Jung (Departments of Physiology and National Research Laboratory for Cellular Signalling, Seoul National University College of Medicine) ;
  • Cho, Ha-Na (Departments of Physiology and National Research Laboratory for Cellular Signalling, Seoul National University College of Medicine) ;
  • Earm, Yung-E (Departments of Physiology and National Research Laboratory for Cellular Signalling, Seoul National University College of Medicine) ;
  • Ho, Won-Kyung (Departments of Physiology and National Research Laboratory for Cellular Signalling, Seoul National University College of Medicine)
  • Published : 2001.04.21

Abstract

L-type $Ca^{2+}$ channels play an important role in regulating cytosolic $Ca^{2+}$ and thereby regulating hormone secretions in neuroendocrine cells. Since hormone secretions are also regulated by various kinds of protein kinases, we investigated the role of some kinase activators and inhibitors in the regulation of the L-type $Ca^{2+}$ channel currents in rat pituitary $GH_3$ cells using the patch-clamp technique. Phorbol 12,13-dibutyrate (PDBu), a protein kinase C (PKC) activator, and vanadate, a protein tyrosine phosphatase (PTP) inhibitor, increased the $Ba^{2+}$ current through the L-type $Ca^{2+}$ channels. In contrast, bisindolylmaleimide I (BIM I), a PKC inhibitor, and genistein, a protein tyrosine kinase (PTK) inhibitor, suppressed the $Ba^{2+}$ currents. Forskolin, an adenylate cyclase activator, and isobutyl methylxanthine (IBMX), a non-specific phosphodiesterase inhibitor, reduced $Ba^{2+}$ currents. The above results show that the L-type $Ca^{2+}$ channels are activated by PKC and PTK, and inhibited by elevation of cyclic nucleotides such as cAMP. From these results, it is suggested that the regulation of hormone secretion by various kinase activity in $GH_3$ cells may be attributable, at least in part, to their effect on L-type $Ca^{2+}$ channels.