Interrelationship between Cell Differentiation and Expression of mRNA for Transferrin in HL-60 Leukemia Cell Line

  • Lee, Soo-Young (Department of Natural Sciences, College of Medicine, The Catholic University of Korea) ;
  • Chi, Chung-Hee (Department of Pathology, College of Medicine, The Catholic University of Korea) ;
  • Kim, You-Mie (Department of Natural Sciences, College of Medicine, The Catholic University of Korea)
  • Received : 2000.03.21
  • Accepted : 2000.05.29
  • Published : 2000.07.31

Abstract

The interrelationship between the differentiation and expression of mRNA for transferrin in the HL-60 leukemia cell line was studied. Transferrin mRNA was expressed in HL-60 leukemia cells and the amount was 50% of that in the positive control cell line, HepG-2 cells. The expression of $T_f$ mRNA in HL-60 cells was not regulated by IL-1, IL-6 and $TNF-{\alpha}$, respectively. The expression of $T_f$ mRNA in the differentiated cells into a granulocyte lineage by DMSO, or all-trans RA, was up-regulated (160-170% of control cells); whereas, the expression was not regulated in the differentiated cells into a macrophage lineage by PMA. These results suggest that the differentiation to a granulocyte lineage of HL-60 leukemia cells appear to be related with the upregulation of transferrin mRNA expression.

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