- Volume 27 Issue 2
The Effect of Polycystic Ovarian Follicular Fluid on Sperm Motility in Human in vitro Fertilization
인간체외수정시술시 다낭성난포종 난포액이 정자의 운동성에 미치는 영향
- Kim, Yeon-Hee (Department of Obstetrics and Gynecology, College of Medicine, Chung-Ang University) ;
- Lee, Sang-Hoon (Department of Obstetrics and Gynecology, College of Medicine, Chung-Ang University) ;
- Hur, Min (Department of Obstetrics and Gynecology, College of Medicine, Chung-Ang University)
- Published : 2000.06.30
Objective: The purpose of this study was to evaluate the effect of polycystic ovarian follicular fluid on sperm motility in human in vitro fertilization (IVF). Methods: From May, 1998 to July, 1999, 55 patients who complained of infertility were involved in this study. We obtained ovarian follicular fluids from the patients by ultrasono-guided aspiration. Subjects were divided into two groups. 20 patients who had polycystic ovarian disease were belong to study group, and 25 patients who had normal ovarian follicular fluid were belong to control group. The follicular fluid dilution was done with Ham's fluid as 10%, 20%, 50%, 100%. The sperm motility was analyzed by CASA at 6hr and 12hr after incubation in follicular fluids. Results: The levels of average path velocity (VAP) in all concentration fluid didn't show significant difference between study and control group. The other parameters including curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), and linerity (LIN) were didn't show any significant difference between both groups. Conclusion: PCOD fluid had seemed to have an adverse effect on the sperm biological function. But, this study showed that PCOD fluid had no different effect on sperm motility with normal follicular fluid.