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Separation of Ether-Soluble Neutral Extract of a Commercial High-Grain Feed Stimulating Hay Intake in Cattle

  • Shahjalal, M. (Department of Animal Nutrition, Bangladesh Agriculture University) ;
  • Dohi, H. (Department of Animal Production, Chugoku National Agricultural Experiment Station) ;
  • Kosako, T. (Department of Animal Production, Chugoku National Agricultural Experiment Station) ;
  • Hayashi, Y. (Department of Animal Nutrition, Bangladesh Agriculture University)
  • Received : 1999.04.14
  • Accepted : 1999.06.02
  • Published : 2000.02.01

Abstract

Appetite stimulant ether-soluble neutral extract of a high-grain compound feed was separated into four fractions with an open column of silica gel chromatograph using four carrier solutions containing n-pentane and diethyl ether as 100:0 (Fraction A), 90:10 (Fraction B), 75:25 (Fraction C) and 0:100 (Fraction D). The stimulative effects of the fractions were examined by comparing the intake of hay (with or without the fractions) in two-choice test bioassays with cattle. The Fractions A, B and C of the neutral extract stimulated (p<0.05) hay consumption in cattle, whereas Fraction D had no effect on selective feeding when compared with the control fraction. Furthermore, Fractions A, B and C had higher (p<0.05) feeding stimulative indices (FSI) than that of the Fraction D. The results suggest that chemical stimulants to increase palatability of hay are present in the neutral Fractions A, B and C of high-grain concentrate.

Keywords

Appetite Stimulant;Neutral Extract;Chromatograph;Grain;Cattle;Separation