Retention of Benzoic Acids in RPLC

역상액체크로마토그래피에서 벤조산류의 머무름

  • Lee, Jae-Seok (Research Institute for Basic Sciences Department of Chemistry, Kyung Hee University) ;
  • Choi, Beom-Suk (Research Institute for Basic Sciences Department of Chemistry, Kyung Hee University)
  • 이재석 (경희대학교 기초과학연구소 자연과학대학 화학과) ;
  • 최범석 (경희대학교 기초과학연구소 자연과학대학 화학과)
  • Received : 1999.12.14
  • Published : 2000.06.25

Abstract

Chromatographic retention mechanism of seven benzoic acids in RPLC were investigated. Inorganic salt is pertinent for the measurement of dead time in benzoic acid. Logarthims of capacity factor (log k') and Hildebrand solubility parameter (${\delta}$) showed proportional relationship which imply the hydrophobic effect of the retention mechanism in RPLC. Enthalpies of solute transfer showed inverse proportion to temperature and organic modifier ratio of the mobile phase. It was found that the S value showed positive slope in plot of log k' vs. volume fraction of water in mobile phase. Free energy change increases with increasing organic volume fraction. The hydrophobicity index, ${\varphi}_0$(organic volume fraction) is inversely proportional to column temperature.

Acknowledgement

Supported by : 한국학술진흥재단

References

  1. J. Chromatoger. Sci. v.15 Horvath Cs;W. R. Malender
  2. 2nd Ed. v.118 The Hydrophobic Effect, Formation of Micelles and Biological Membranes C. Tanford
  3. J. Chromatogr v.461 F. Dondi;Y. D. Kahie
  4. J. Chromatogr v.296 X. Geng;F. E. Regnier
  5. J. Chromatogr. A v.71 P. J. Schoemarkers;R. Tihssen
  6. J. Chromatogr v.282 P. J. Schoenmarkers;H. A. H. Billiet;L. de Galan
  7. J. Chromatogr v.318 W. R. Melander;C. A. Mannan;SC. Horvath
  8. J. Chromatogr. v.633 N. Chen;Y. Zhang;P. Lu
  9. Anal. Chem. v.64 L. A. Cole;J. G. Dorsey;K. A. Dill
  10. Anal. Sci & Tech. v.11 Y. J. Moon;S. W. Kang
  11. J. Chromatogr. v.179 L. R. Snyder
  12. Anal. Chem. v.54 P. Jandera;H. Colin;G. Guiochon
  13. J. Chromatogr. v.318 W. R. Malender;B. K. Chen;Cs. Horvath
  14. J. Chromatogr v.606 N. Chen;Y. Chang;P. J. Lu
  15. J. Kor. Chem. Soc. v.41 no.1 E. A. Yoo;S. Y. Jung
  16. J. Chromatogr v.631 K. Valko;P. Stegel