Preconcentration and Speciation of Trace Mercury Compounds in Water Sample Using Dithizonates Extraction and Reverse Phase Liquid Chromatography

디티존 착물 추출과 역상 액체 크로마토그래피를 이용한 물시료 중의 미량 수은 화합물의 농축 및 화학종의 분리

  • Suh, Jung-Ki (Division of Chemical Metrology and Materials Evaluation, Korea Research Institute of Standards and Science) ;
  • Cho, Kyung-Haeng (Division of Chemical Metrology and Materials Evaluation, Korea Research Institute of Standards and Science) ;
  • Lee, Sang-Haak (Department of Chemistry, Kyungpook National University)
  • 서정기 (한국표준과학연구원 물질량표준부) ;
  • 조경행 (한국표준과학연구원 물질량표준부) ;
  • 이상학 (경북대학교 자연과학대학 화학과)
  • Received : 1999.07.28
  • Published : 2000.02.25

Abstract

A rapid preconcentration method was developed for the speciation of the trace mercury compounds in water sample. The mercury compounds were extracted and preconcentrated simply as their dithizone complexes by passing through the dithizone impregnated ultra-high molecular weight polyethylene (UHMWPE) membrane solvent inlet filter following sanification in methanol solvent. The concentrated dithizonates were separated by liquid chromatography on a $C_{18}$ column. Complete resolution was obtained between methyl-, ethyl-, phenyl-, and inorganic mercury with a mobile phase of 0.05 M acetate buffer (pH=4)/THF/methanol(3:5:2). The separnted mercury chelates were detected by spectrophotometrically at 475 nm. The proposed method was successfully applied to the speciation of mercury compounds in waste water with detection limit at the subnanogram/mL level.

Keywords

merctuy;speciation;dithizone;water;membrane filter

References

  1. Adv. Chromatogr. v.22 H. Veening;B.R. Willeford.
  2. Analyst. v.11 I.S. Krull;D.S. Bushee;R.G. Schleicher;S.B. Smith
  3. J. Chromatogr. v.252 S.R. Hutchins;P.R. Haddad;S. Dilli
  4. Analyst(London) v.102 Analytical Methods Committee
  5. J. Chromatogr. v.76 G.L. Baughman;M.H. Carter;N.L. Wolf;R.G. Zepp
  6. Anal. Chim. Acta v.140 M. Goto;T. Shibakawa;T. Arita;D. Ishii
  7. Mercury in the environment L. Friberg;J. Vostal
  8. J. Chromatogr. Sci. v.19 D.E. Henderson;R. Chaffee;F.P. Novak
  9. Anal. Chim. Acta v.45 H. Irving;A.M. Kiwan
  10. Anal. Chem. v.47 R. Litman;A.L. Finston;E.T. Williams
  11. Anal. Chem. v.58 I.T. Urasa;F. Ferede
  12. Anal. Chem. v.48 A.A. El-Awady;R.B. Miller;M.J. Carter
  13. Anal. Chim. Acta. v.185 W. Langseth
  14. J. High Resolut. Chromatogr. Commun. v.8 P. Bruno;M. Caselli;A. Traini
  15. Z. Anal. Chem. v.326 R. Ahmed;K. May;M. Stoeppler;Fresenius