The inhibition of chitin synthesis in Spodoptera litura by new insecticides of benzoylphenyl urea, DBI-1015 and DBI-3204

담배거세미나방(Spodoptera litura)에서 benzoylphenyl urea계의 신규살충제 DBI-1015 및 DBI-3204의 키틴합성 저해 효과

  • Song, Cheol (Agrochemical Research Team, Korea Research Institute of Chemical Technology) ;
  • Shin, Wook-Kyun (Agrochemical Research Team, Korea Research Institute of Chemical Technology) ;
  • Cho, Kwang-Yun (Agrochemical Research Team, Korea Research Institute of Chemical Technology)
  • 송철 (한국화학연구소 농약활성연구팀) ;
  • 신욱균 (한국화학연구소 농약활성연구팀) ;
  • 조광연 (한국화학연구소 농약활성연구팀)
  • Published : 2000.06.30

Abstract

This study was conducted to investigate insecticidal mechanisms of the new insecticides DBI-1015 and DBI-3204 with label compounds of chitin precursors, [$^{14}C$] N-acetylglucosamine and [$^{14}C$] UDP-N-acetylglucosamine in Spodoptera litura. The concentrations of the insecticides for incorporation of chitin precursors into chitin were founded to be functional relationship. The result of in vivo test, $I_{50}$ (ppm) of the DBI-1015, DBI-3024 and diflubenzuron to [$^{14}C$] N-acetylglucosamine were 0.57, 0.89 and 0.26 ppm respectively, and to [$^{14}C$] UDP-N-acetylglucosaminen were 0.99, 0.53 and 0.45 ppm respectively. in vitro test of DBI-1015, DBI-3024 and diflubenzuron by integument fragments, the incorporation rate in the cuticle were low, however, $40{\sim}60%$ inhibitions were observed at $2{\mu}M$ when compared to the untreated control.

Keywords

Insecticide;chitin precursor;N-acetylglucosamine;UDP-N-acetylglucosamine;diflubenzuron