Inhibitory Effects of Asadisulphide on the TEA-induced Adherence of HL-60 Cells

TPA로 야기된 HL-60 세포의 기질부착에 대한 Asadisulphide의 억제 효과

  • 유관희 (충남대학교 자연과학대학 생물학과) ;
  • 박미아 (충남대학교 자연과학대학 생물학과) ;
  • 김선희 (충남대학교 약학대학 약학과) ;
  • 안병준 (충남대학교 약학대학 약학과)
  • Published : 2000.09.01

Abstract

Asadisulphide were purified from Ferrula assafoetida by organic solvent extraction and chromatography. Its inhibitory effects on the TPA-induced adherence of HL-60 cells was analyzed. Since ethyl acetate extracts of F. assafoetida has the strongest inhibitory effect on adherence of HL-60 cells, it was re-extracted with ethyl acetate, hexane, and ethyl ether and chromatographed three times to isolate asadisulphide. At the minimum concentration of 2 $\mu\textrm{g}$/ml, asadisulphide inhibited adherence of 98% of HL-60 cells that have been treated with TPA. It also showed anti-cancer effect with no cytotoxity in the ED$_{50}$ value of 20 $\mu\textrm{g}$/ml.