Optimization of D-\beta-hydroxybutyric Acid Fermentation Using a Mutant of Candida Rugosa IFO0750

Candida rugosa 변아주를 이용한 D-\beta-Hydroxybutyric Acid 발효공정의 최적화

  • 경수현 (연세대학교 공과대학 생명공학과) ;
  • 신철수 (연세대학교 공과대학 생명공학과)
  • Published : 2000.12.01


A UVmutant of Candida rugosa IF00750 was made and used to convert butYlic acid to D-$\beta$-hydroxybutyric acid(D-$\beta$-HBA). Major regulating factors for D-$\beta$-HBA fennentation were investigated via chemostat analyses. The maximum specific productivity was achieved at a specific growth rate of $0.06h^{-1}$ where the glucose and butyric acid concentrations in the fermentor were 10 g/L and 8.7 g/L. respectively. A fed-batch fennentation was performed with maintenance of the optimum glucose and butyric acid concentrations. The D-$\beta$-HBA concentration after 120 h of cultivation reached 12.4 g/L, which was 4.7 times greater illan the concentration obtained by batch fermentation.


Candida rugosa;fermentation;D-\beta-hydroxybutyric acid


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