Optmization of Culture Conditions and Nitrogen Sources for Production of Erythritol by Candida magnoliae.

Candida magnoliae에 의한 에리스리톨 생산을 위한 최적 배양환경과 질소원 선별

  • 고은성 (서울대학교 협동과정 생물화학공학 전공) ;
  • 문관훈 (서울대학교 식품공학과) ;
  • 한기철 (서울대학교 식품공학과) ;
  • 유연우 (아주대학교 분자과학기술학과) ;
  • 서진호 (서울대학교 식품공학과)
  • Published : 2000.12.01

Abstract

Culture conditions and nitrogen sources were optimized for production of erythritol, a natural sweetener, by Candida magnoliae M26. The optimal culture conditions were found to be culture temperature of $28^{\circ}C$, initial pH of 7, aeration of 1 vvm and agitation speed of 500 rpm in a 2.5 1 jar-fermentor. Glucose was chosen as the best carbon cource bsed on cell growth and erythritol productivity. Kight steep water(LSW) and corn steep liquor (CSL) which are by-products in starch processing from corn were tested as a nitrogen source substitute for yeast extract. The use of either LSW or CSL did not change the fermentation performance. The experimental results using LSW and CSL showed 1.5 times higher in cell growth and almost the same value in erythritol productivity com-pared with the control fermentation using yeast extract as a nitrogen source. These results suggested that either LSW of CSL could be used as a nitrogen source in a large-scale fermentation for erythritol production.

Keywords

Candia magnoliae;erythritol;culture condition;optimization;light steep water;corn steep liquor

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