Characterization of Extracellular Protease of Bacillus sp. WRD-1 Isolated from Soil

토양으로부터 분리한 Bacillus sp. WRD-1이 생산하는 Extracellular Protease의 특성

  • Ok, Min (Division of Biotechnology, Faculty of Natural Resource and Life Science, Dong-A University) ;
  • Kim, Min-Seok (Division of Biotechnology, Faculty of Natural Resource and Life Science, Dong-A University) ;
  • Seo, Won-Seok (Division of Biotechnology, Faculty of Natural Resource and Life Science, Dong-A University) ;
  • Cha, Jae-Young (Division of Biotechnology, Faculty of Natural Resource and Life Science, Dong-A University) ;
  • Cho, Young-Su (Division of Biotechnology, Faculty of Natural Resource and Life Science, Dong-A University)
  • 옥민 (동아대학교 생명자원과학부 생물공학전공) ;
  • 김민석 (동아대학교 생명자원과학부 생물공학전공) ;
  • 서원석 (동아대학교 생명자원과학부 생물공학전공) ;
  • 차재영 (동아대학교 생명자원과학부 생물공학전공) ;
  • 조영수 (동아대학교 생명자원과학부 생물공학전공)
  • Published : 2000.12.01

Abstract

Alkaline bacterium producing a high pro-tease activity at low temperature was isolated by using enrichment culture from soil samples and identified as Bacil-lus sp. WRD-1 Cell growth was maximal at 10 hours and the optimal initial pH and culture time of culture condition for enzyme production was pH 7 and 10 hours, respectively. Temperature range of high enzyme activity were $10~40^{\circ}C$. The optimal pH and temperature for the enzyme activity were pH9 and $30^{\circ}C$.

Keywords

Bacillus sp.;WRD-1;protease activity;low temperature

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