Prevalence of Salmonella Enterotoxin Gene(stn) among Clinical Strains Isolated in Korea and Regulation of stn Expression

한국에서 분리된 병원성 Salmonella 균주의 장독소 유전자(stn) 분포와 발현조절 기작

  • 임상용 (서울대학교 농생명공학부 식품공학과) ;
  • 유상렬 (서울대학교 농생명공학부 식품공학과)
  • Published : 2000.12.01

Abstract

Role of enterotoxin from Salmonella in pathogenesis is not know. Enterotoxin gene from Salmonella typhimurium(stn) encodes a 29kDa toxin that has no homology to any other known enterotoxins. Expression of stn is enthanced upon contact with epithelial cell but not all strains having the stn gene express Stn, Based on PCR analysis, we found that all 36 clinical strains of Salmonella isolated in Korea tested carried the stn gene. To understand the trgulation of the stn transcription, the expression of stn was studies in vitro. RNA polymerase was purified by polymin P fraction-ation, DNA-agarose affinity chromatography, and Mono-Q ion exchange chromatography from Salmonella. The expression of stn was inhibited by cAMP·CRP complex by about 50%.

Keywords

Salmonella enterotoxin(stn);PNA polymerase purification;cAMP·CRP complex

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