Isolation and Indentification of Yeast Strains Producing High Concentration of Ethanol with High Viabi-lity.

에탄올 생성능과 생존능이 우수한 효모균주의 분리와 동정

  • Kang, Tae-Young (Department of Genetic Engineering, The University of Suwon) ;
  • Oh, Gui-Hwan (Department of Genetic Engineering, The University of Suwon) ;
  • Kim, Keun (Department of Genetic Engineering, The University of Suwon)
  • 강태영 (수원대학교 유전공학과) ;
  • 오귀환 (수원대학교 유전공학과) ;
  • 김근 (수원대학교 유전공학과)
  • Published : 2000.12.01

Abstract

To isolate yeast strains producing high concentration of ethanol, 125 strains were subjected to screening. Initially 14 strains able to grow in a medium containing 15%(v/v) ethanol, 7 strains capable of growing in a medium containing 50%(v/v) glucose, 23 strains having relatively fast fermentation rates, 13 strains able to grow at $42^{\circ}C$ were selected. After secondary screening, 11 strains having relatively high ini-tial fermentation rate and producing high concentration of ethanol were selected. After tertiary screening 5 strains producing high concentration of ethanol were selected. These 5 strains were again for their ethanol produc-tion, residual sugar, and viability using fermentation medium containing 25% glucose. The strain producing the highest concentration of ethanol was 20-1 strain which produced 10.56%(v/v) ethanol in 4 days, and the highest viable strain was 11-1 which produced 10.35%(v/v) ethanol(13.1%. v/v) with the viability of 30.44% after 5 days of fermentation. Both of the 20-1 and 11-1 strains were identified as Saccharomyces cerevisiae.

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