The Role of Vitamin C and Vitamin E Supplementation on Iron Contents and Biomarkers of Oxidative Stress in Blood, Liver and Brain of Aging Rats

나이에 따른 흰쥐의 혈액, 간, 뇌조직의 철분함량, 산화 스트레스 지표에 대한 비타민 C 와 비타민 E공급의 역할

  • 황은희 (원광대학교 생활과학대학)
  • Published : 2000.07.01

Abstract

The purpose of this study was to investigate the effect of vitamin C and vitamin E supplementation on the iron contents and oxidative stress of the rats. Rats were fed 18g ascorbic acid and 300IU $\alpha$-tocopherol/kg diet, respectively. Rats were sacrificed at 1, 3, 5 and 7 month of age. The blood, liver and brain were selected for the quantitation of iron and malondialdehyde(MDA) contents, glutathione peroxidase(GSHPx), superoxided dismutase(SOD) and catalase(CAT) activity. Iron and MDA contents and GSHPx activities were increased with aging. Vitamin C and Vitamin E supplementation increased iron contents of the plasma. Vitamin C raised iron contents, but vitamin E decreased iron contents of the liver. In the brain vitamin C and vitamin E did not affect the iron level. MDA levels were decreased with vitamin C and vitamin E supplementation in the erythrocyte and liver, and vitamin C supplementation elevated MDA levels in the brain. GSHPx activity was increased with vitamin C and vitamin E supplementation. SOD activities of erythroucyte and brain were not affected with age, but in the liver, SOD activity was raised with age and vitamin C supplementation. Vitamin C and vitamin E supplementation promoted CAT activity of erythroucyte and liver, and CAT activity of brain was eleveated with vitamin addition but was decreaed with vitamin E addition. Vitamin C and vitamin E decreased iron contents of blood plasma, MDA contents of plasma and liver, and CAT activity of erythrocyte. Above results indicated that iron contents and biomarkers of oxidative stress were more affected by age than antioxidant action of vitamin C and vitamin E.

References

  1. Present Knoledge in Nutrition(7th Edi) Ziegler EE;Filer LJ
  2. Iron and Human Disease Lauffer RB
  3. Advanced Nutrition Kim SH
  4. Kr J Nutr v.28 no.3 Is there a need for an estimate of an upper safe limit of nutrient intake? Chang NS
  5. Kr J Nutr v.32 no.3 Effect of calcium and iron loading on bioavailability of minerals in normal and Ca/Fe deficient rats Lee YS;Lee JH
  6. Kr J Nutr v.30 no.3 Prevent effect of selenium supplementation on iron accululation of rats fed diet containing high levels of iron Jun YS
  7. J Clin Invest v.86 Molonaldehyde and 4-hydroxynonenal protein adducts in plasma and liver of rats with iron over load Houglum KM;Filip JL;Wigztum and M Chojkier
  8. Trends in Biochemical Science v.11 Iron and radical reaction: two aspects of antioxidant protection Halliwell B;Gutteridge J
  9. Tissue iron overload and mechanism of iron catalyzed oxidative injury free radicals in diagnostic medicine Lesnefsky E;D Armstrong(ed.)
  10. J Biol Chem v.259 Iron catalyzed hydroxylradical formation formation Graf E;Mahoncy JR;Bryant RG;Eaton JW
  11. Hepatology v.5 Hepatic mitochondrial oxidative metabolism in rats with dietary iron overload Bacon BR;Park CH;Brittenham G;O'Neil R;Travil AS
  12. J Lab Clin Med v.121 Iron loading of cultured cardiac myocytes modifies sarcolemmal stucture and increases lysosomal fragility Link G;Pinson A;Hershko C
  13. Ann Neurol v.32 Studiesof the mechanism of iron transpot accross the blood-brain barrier Roberts R;Sandra A;Seik GC;Lucas JJ;Fine RE
  14. J Neurochem v.63 no.4 Alterd brain metabolism of iron as a cause of neurodegenerative disease? Gerlach M;Ben-Shachar D;Riedrer P;Youdim BH
  15. Acta Neurol Scand v.87 no.S146 Altered mitochondrial funtion, iron metabolism and gletathione levels in Pakinson's disease Jenner P
  16. J Neuroscience Research v.31 Regional distribution of iron and iron regulatiry proteins in the brain in aging and Alzheimer's disease Connor JR;Snyder BS;Beard JL;Fine RE;Mufson EJ
  17. Biological Trace Element Reseach v.34 Roles of iron in neoplasia Weinberg ED
  18. Free Radical Biol Med v.12 Dietary iron and colorectal cancer risk Nelson RL
  19. Free Radicals in Aging Yu BP
  20. Biochemica et Biophysica Acta v.1275 The ferritin: molecular properties, iron storage function and celluar regulation Harrison PM;Arosio P
  21. J Neurochem v.63 Iron, transferrin, and ferritin in the rat brain during development and aging Roskams AJ;Connor JR
  22. J Gerontol v.45 In memorial Nathan W. Shock 1906-1989 Christofalo VJ;Bajer GT
  23. Free Radical Biology & Medicine v.21 Aging, and oxidative stress Yu BP
  24. J Nutr v.126 Vitamin C-driven free radical generation from iron Herbert V;Shaw S;Jayatilleke
  25. J Biological Chem v.261 no.30 The effect of effects of ascorbic acid on the intracellular metabolism of iron and ferritin Bridges KR;Heffman KE
  26. Biochem J v.241 Superoxide-dependent and ascorbate-dependent formation of hydroxy radicals from hydrogen peroxide in the presence of iron Aruoma OJ;Halliwell B
  27. J Biol Chem v.262 no.30 Ascorbic acid inhibits lysosomal autopagy of ferritin Bridgeo KR
  28. Am J Clin Nutr v.53 Antioxidant nutrients and disease prevention Diplock AT
  29. Annu Rev Nutr v.12 Dietary carotenes, Vitamin C and Vitamin E as protective antioxidants in human cancers Byers T;Perry G
  30. N Engl J Med v.328 Vitamin E consumption and the risk of coronary heart disease in men Rimm EB;Stampfer MJ;Ascherio A;Giovannucci E, Colditg GA;Willett WC
  31. Anal Biochem v.72 A rapid and sensitive method or the quantitation of microorganism quantities of protein utilizing the principle of protein-dye binding Bradford MM
  32. Am J Clin Path v.37 Chemical and diagnostic specificity of laboratory tests Wendell, TC
  33. Meth Enzymol v.158 Rapid Colorimetric micromethod for the quantiation of complexed iron in biological samples Fish W
  34. Free Radical Biology and Med v.9 Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury Janero DR
  35. J Lab Clin Med v.70 Studies on the quantitative and qualitative characterization of erythrocyte gluathione peroxidase Paglia DE;Valentine WN
  36. Meth Enzymol v.105 Assay of glutathione peroxidase Flohe L;Gunzler WA
  37. CRC Handbook of Free Radicals and Antioxidants Biomedicine Convenient assay for superoxide dismutase Flohe L;Brigelius R;Lengfelder E;Otting F
  38. Analytical Biochem v.174 A spectrophotometric method for determination of catalase activity in small tissue sample Johnsson LH;Haken Bong LA
  39. Kr J Nutr v.32 Effects of vitamin E and vitamin C supplementation on the decrease of cognitive function induced by Scopoline Ahn HS;Kang SA;Lee IH
  40. Kr J Nutr v.30 Effect of excess vitamin C feeding on blood and liver lipid and its peroxidation levels and platelet thromboxane A₂formation in rats Lee JW;Kim SY;Kwak CS
  41. Free Radi Biol Med v.17 no.2 Dietary vitamin C decreases endogenelous protein oxidative demage, malondialdehyde and lipid peroxidation and maintains fatty acid unsatuation in the guinea pig liver Barja G;Lopez-Torres M;Perez-Campo R;Rojas C;Cadenas S;Prat J;Pamplona R
  42. J Natr v.103 Effect of large dose of vitamin C in guinea pigs and rats Nandi BK;Majumber AK Subramanian N;Chatterjee I
  43. Mechanisms of Aging and Develoment v.102 Iron accumulation in aging modulation by dietary restriction Cook CI;Yu BP
  44. Biochem and Biophys Res Comm v.78 Age related changes in lipid peroxidation in rat brain and liver Sharma OP
  45. J Lab Clin Med v.129 Non-Heme $(Fe^{3+})$ in the lung increases with age in both human and rats Ghio AJ;Pritchard RJ;Dittrich KL;Samat JM
  46. Biochem Biophys Acta v.1275 The ferritin molecular properties iron storage function and cellular regulation Harrison PM;Arosio P
  47. Biochem v.36 Dinuclear center of ferritin: studies of iron binding and oxidation show difference in two iron sites Treffy A;Zhao Z;Quail MA;Guest JR;Harrison PM
  48. Kr J Nutr v.31 The effect of weekly iron supplementation on iron and zink nutritional status in pregnant women YU KH;Yoon JS
  49. Kr J Nutr v.31 Aanlysis of factors to influence requirement of vitamin E and vitamin C in young and healthy men and women Park S;Yu JG;Lee UJ
  50. Kr J Nutr v.32 no.7 Effects of antioxidant vitamins supplementtation on antioxidative status and plasma lipid profile in Korean NIDDM patients Kang NE;Kim WK
  51. J Neurochem v.67 Ascorbate-stimulated lipid peroxidation in human brain is dependent on iron but not on hydroxyl radical Andron AC;Britton RS;Bacon BR
  52. J Free Radic Biol Med v.1 Mechamisms of lipid peroxidation Girotti A
  53. J Clin Invest v.82 Hemoglobin mediated oxidant demage to the central nervous system requires endodeneous ascorbate Sadzadeh SM;Eaton JW
  54. Kr J Nutr v.31 no.2 Effects of vitamin E supplementation on the lipid peroxides and activiies of antioxidative enzymes in the pancreas of diabetic KK mice Chang YS;Ahn HS;Kim H
  55. Kr J Nutr v.32 no.4 Effect of dried leaf powders and ethanol extracts of Persimmon, Green Tea and Pine needle on lipid methbolism and antioxidative capacity in rats Kim ES;Kim MK