Detection of Irradiated Beans Using the DNA Comet Assay

DNA Comet Assay를 이용한 콩류의 방사선 조사 확인

  • 오경남 (한국원자력연구소 식품검지실) ;
  • 김경은 (한국원자력연구소 식품검지실) ;
  • 양재승 (한국원자력연구소 식품검지실)
  • Published : 2000.10.01

Abstract

The single cell-gel electrophoresis assay (comet assay) was used to identify irradiated beans. Soy beans, kidney beans, and red beans were irradiated with $^{60}Co$ gamma rays at 0.1, 0.3, 0.5, 0.7, and 1.0 kGy. Beans were peeled out, crushed lightly, and treated with phosphate-buffered saline (PBS) to extract cells. The extracted cell suspension was mixed with agarose gel solution and spread on an agarose precoated slide. After lysis of the cells, they were subjected to microgel electrophoresis for 2 minutes, and then silver-stained. We found that the DNA fragments of the irradiated samples were stretched, migrated out of the cells, and formed tails towards the anode giving the appearance of comets, while the unirradiated or the undamaged cells formed very short or no tails. The tail lengths of irradiated samples were significantly increased as irradiation dose increased at the above 0.3 kGy.

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