Inhibitory Effects of Various Edible Plants and Flavonoids from the Leaves of Cedrela sinensis on Human Immunodeficiency Virus Type 1 Protease

  • Park, Jong-Cheol (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Hur, Jong-Moon (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Park, Ju-Gwon (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Kim, Hyun-Joo (Department of Cosmetology, Sunchon Chongam College) ;
  • Kang, Kyeong-Hee (Department of Cosmetology, Sunchon Chongam College) ;
  • Park, Myeong-Rak (Department of Biotechnology, Yosu National University) ;
  • Song, Sang-Ho (Department of Biotechnology, Yosu National Universit)
  • Published : 2000.09.01

Abstract

The inhibitory effect of extracts from 15 edible plants on the protease of human immunodeficiency virus (HIV) type 1 was investigated. Protease activity was determined by incubating the extracts in a reaction mixture containing protease and substrate His-Lys-Ala-Arg-Val-Leu-(p-NO$_2$-Phe)-Glu-Ala-Nle-Ser-NH$_2$ to inhibit proteolytic cleavage. Of various plants tested, the leaves of Cedrela sinensis inhibited the HIV-1 protease by 42% at a concentration of 100$\mu\textrm{g}$/ml. A major flavonoid isolated from the leaves of C. sinensis, quercetin 3-O-$\alpha$-L-rhamnoside showed inhibitory activity of 19% at a concentration of 100$\mu$M.