Enhancement of Xylitol Yield by Xylitol Dehydrogenase Defective Mutant of Pichia stipitis

Pichia stipitis의 Xylitol Dehydrohenase Defective Mutant에 의한 Xylitol 수율 향사

  • Kim, Min-Soo (Department of Molecular Science and Technology, Ajou University) ;
  • Kim, Chul (School of Chemical Engineering and Biotechnology, College of Engineering, Ajou University) ;
  • Seo, Jin-Ho (Department of Food Science and Technology, Seoul National University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University)
  • 김민수 (아주대학교 대학원 분자과학기술학과) ;
  • 김철 (아주대학교 대학원 화학생물공학부) ;
  • 서진호 (서울대학교 식품공학과) ;
  • 유연우 (아주대학교 대학원 분자과학기술학과)
  • Published : 2000.04.01

Abstract

In order to produce xylitol with high yield, experiments were carried out to develope xylitol dehydrogenase (XDH) defective m mutant from Pichia stipitis and to investigate the xylit이 fermentation characteristics of mutant strain. After treatment of P s stipitis with EMS, mutant PXM-4 was selected based on the XDH activity and xylitol production capability. Among the tested c cosubstrates, galactose was selected as an adequate cosubstrate on xyl뻐I production of mutant PXM-4. With the increase of galactose concentration, xylitol production was decreased because the transport of xylose into cell was inhibited by g galactose. The optimal concentration of galactose for the production of xylitol using 20 g/L xylose was 20 g/L. Under this c condition, maximum concentration of xylitol and yield were 14.4 g/L and 97%, respectively. In order to prevent the inhibitory e effect of xylose transport by galactose, galactose was fed with low concentration and the concentration of xylitol produced w was increased up to 25 g/L. In the fermentation of corn cob hydrolyzate by mutant PXM-4, xylose was completely converted t to xylit이 with a 100% yield in 4 days culture.

References

  1. Agric. Biol. Chem. v.30 The Production of xylitol, L-arabirol and ribitol by yeasts Onishi H.;T. Suzuk
  2. J. Ind. Microbiol. v.3 Screening of teasts for production of xylitol from D-xylose and some factors which affect xylitol yield in Candida guilliermondii Barbosa M .F. S.;M. B. Medeiros;I. M. de Mancilha;H. Schnieder;H. Lee
  3. Biotech. Bioeng. v.40 Production of xylitol from xylose by C. tropicalis: Optimization of production rate Horitsu H.;Y. Hayashi;K. Takamizawa;K. Kawai;T. Suzuki;N. Wadanabe
  4. Biotechnol. Lett. v.13 Study of xylitol formation from xylose under oxygen limiting conditions Furlan S. A.;P. Bouilloud;P. Strehaiano;H. F. de Castro
  5. Biotechnol. Lett. v.17 The reduction of xylose to xylitol by C. guilliermondii and C. parapsilosis: Incidence of oxygen and pH Nolleau V.;L. Preziosi-Belloy;J. M. Navarro
  6. Biotechnol. Lett. v.18 Xylitol formation by Candida boidinii in oxygen limited chemostat culture Winkenlhausen E.;P. Pittman;S. Kuzmanova;T. W. Jeffries
  7. Process Biocehm. v.31 Fed-batch culture for xylitol production by C. guilliermondii Silva, S. S.;I. C. Roberto;M. G. A. Felipe;I. M. Mancilha
  8. J. Ferment. Bioeng. v.81 no.2 Production of xylitol from D-xylose by Candida tropicalis: The effect of D-glucose feeding Yahashi, Y.;H. Horitsu;K. Kawai;T. Suzuki;K. Takamizawa
  9. J. Microbiol. Biotechnol. v.3 no.2 Selective isolation and characterization of Schwanniomyces castellii mutants with increased production of α-amylase and glucoamylase Ryu Y. W.;S. H. Ko
  10. Kor. J. Biotechnol. Bioeng. v.13 no.1 Priduction of xylitol by cataboilte derepressed mutant of Candida sp Han W. O.;J. H. Seo;Y. W. Ryu
  11. J. Biochem. v.193 Protein measurement with the Folin phenol reagent Lowry O. H.;N. R. Rosebrough;A. L. Farr;R. G. Randall
  12. Biotech. Bioeng. v.54 Fed-batch xylitol production with two recombinant Saccharomyces cerevisiae strains expressing xyll at different levels, using glucose as a cosubstrate: a comparison of production parameters and strain stability Nina Q. M.;H. Babel
  13. J. Gen. Microbiol. v.132 Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae Rusturia, A.;R. Lagunas
  14. J. Gen. Microbiol. v.129 An enzyme analysis of NADPH production and consumption in C. utilis Bruinenberg P. M.;J. P. van Dijken;W. A. Scheffers
  15. W. J. Microbiol. Biotechnol. v.11 Effects of environmental conditions on production of xylitol by Candida boidinii Vandeska E.;S. Amartey;S. Kuzmanova;T. W. Jeffries
  16. Biotechnol. Lett. v.13 Xylitol production from D-xylose by Candida guilliermondii : Fermentation behaviour Meyrial, V.;J. P. Delgenes;R. Moletta;J. M. Navano