Enhancement of Xylitol Yield by Xylitol Dehydrogenase Defective Mutant of Pichia stipitis

Pichia stipitis의 Xylitol Dehydrohenase Defective Mutant에 의한 Xylitol 수율 향사

  • Kim, Min-Soo (Department of Molecular Science and Technology, Ajou University) ;
  • Kim, Chul (School of Chemical Engineering and Biotechnology, College of Engineering, Ajou University) ;
  • Seo, Jin-Ho (Department of Food Science and Technology, Seoul National University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University)
  • 김민수 (아주대학교 대학원 분자과학기술학과) ;
  • 김철 (아주대학교 대학원 화학생물공학부) ;
  • 서진호 (서울대학교 식품공학과) ;
  • 유연우 (아주대학교 대학원 분자과학기술학과)
  • Published : 2000.04.01


In order to produce xylitol with high yield, experiments were carried out to develope xylitol dehydrogenase (XDH) defective m mutant from Pichia stipitis and to investigate the xylit이 fermentation characteristics of mutant strain. After treatment of P s stipitis with EMS, mutant PXM-4 was selected based on the XDH activity and xylitol production capability. Among the tested c cosubstrates, galactose was selected as an adequate cosubstrate on xyl뻐I production of mutant PXM-4. With the increase of galactose concentration, xylitol production was decreased because the transport of xylose into cell was inhibited by g galactose. The optimal concentration of galactose for the production of xylitol using 20 g/L xylose was 20 g/L. Under this c condition, maximum concentration of xylitol and yield were 14.4 g/L and 97%, respectively. In order to prevent the inhibitory e effect of xylose transport by galactose, galactose was fed with low concentration and the concentration of xylitol produced w was increased up to 25 g/L. In the fermentation of corn cob hydrolyzate by mutant PXM-4, xylose was completely converted t to xylit이 with a 100% yield in 4 days culture.


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