Improving Detection Method of Pseudomonas aeruginosa, an Important Index Organism of Bottled Water Quality

먹는샘물의 녹농균 분포 및 검사방법 개선에 관한 연구

  • 정현미 (국립환경연구원 수질연구부 수질 미생물과) ;
  • 김동빈 (국립환경연구원 수질연구부 수질 미생물과)
  • Published : 2000.09.01


Since the official allowance of bottled water at Korean domestic market in 1995, Pseudomo~zas aemginosu has been detected from 2.3% and 1.2% of source and products of bottled water sa~nple tested, respectively. according to the nation-wide dala froin May 1995 to December 1996. Therefore, P aeivginosa was the second most important parameter, next to colifoi~ns, anlong the bacieriological parameters regulated for bonled water. The official standard method initially adopted the Japanese officlal method and Standard Methods of the US, w~hich is using aspai-agiii-acetamid mnedia(A-A method). how eve^; the method showed low specificity regardless of the high sensitivity. The $42^{\circ}C$ growth test was the best biochemical featu1-e differentiating the P uelarginosu 6-om P aei-uginosa-like species such as P puririir and P Jhoi.escens amo1zg the other characierisiics such as fluorescence pigment_ pyocyanin, casein hy&olysis, etc. Thel-efore, addition of the $42^{\circ}C$ growth Lest in advance ofthe biochemical identification test, when sainple is positive by A-A method, should strengthen the spec~IiclQ w~tli ~ninin~um addition of testing load.


Pseudomonas(P.) aeruginosa;bottled water;official detection methods;recovery;sensitivity;specificity


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