Overproduction of Lignin Peroxidase from Phanerochaete chrysosporium PSBL-1

Phanerochaete chrysosporium PSBL-1의 배양조건 최적화를 통한 Lignin Peroxidase의 과량생산

  • 정병철 (명지대학교 생명과학과/환경생명공학원) ;
  • 한윤전 (명지대학교 생명과학과/환경생명공학원) ;
  • 장승욱 (명지대학교 생명과학과/환경생명공학원) ;
  • 정욱진 (화학공학과 /청정기술원) ;
  • 원유정 (화학공학과/인수환경)
  • Published : 2000.09.01

Abstract

Until now, it was diIliculi to overproduce lignin peroxidase(LiP) fiom Pl~anemchaete ch~ysosporium since the lack of optimized growth conditions. In this paper, we optimized the LIP production conditions and monitored LIP isozyines of fl chqsospoi.ium PSBL-1. The optimized condition includes sponge matrix support, no addition of $MnSO_4$, excess addition of niixogen source(48 inM diarmnonium), and addition of stabilizer(2 mM verakyl alcohol). Finally we obtained Lip activity of 1,800 unitsll. HI isozyne was overproduced when inyceliuin was cultivated in media containing $Mn^{2+}$ (2.73 inM) and excess nitrogen(48 11d4 diannnonium). Three azo dyes(acid yellow 9, congo ued, orange IT; each concenimtion of50 $\mu$M) we1-e rapidly decolorized within 2 inins by 0.4 un~t or Lip.

Keywords

isozyme profile;lignin peroxidase over production;manganese deficient culture;Phanerochaete chrysosporium PSBL-1

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