Coagulase Thping and Antibiotic Resistance of Methicillin Resistant Staphylococcus aureus (MRSA) Isolated form Patients in Pusan

부산지역 환자로부터 분리된 Methicillin Resistant Staphylococcus aureus(MRSA)의 응고효소형 및 항균제 내성에 관한 연구

  • 류지한 (부경대학교 미생물학과) ;
  • 이훈구 (부경대학교 미생물학과)
  • Published : 2000.09.01


Eighty-eight strain3 o~methicillin resistant Stopllylococcus awecis were Isolated from pus (64.7%); spuhm (26.2%), blood, fluid, andurine of 83 patients at Dong-A Hospital in P~~san to invesligate theil-coagulase typ- Ing, and multi-drug resistaut ppattems. The presence of niec A gene confe~~ing melhicillin resistance was tested by polymerase chain reaction (PCR) with uwo mec A gene specific primers using purified clromosonlal DNA as templates. DNA fragments of expected size wel-e detected frorn 86 strains, but not from two strains. !i coagulase typmg, the 86 isolates were assigned to 5 coagulase lypes, I, 11, lll. 1V, VI, VII, VIlI, but there was no isolate helong lo type V. The most abundant coagulase type was type TI(50 %), lollowed by type IV Rest ofthe coagulase types were ininor; ranging fmm 4.5 to 12.5 '% Most of the type I1 ~netlucillin resistant Stapl\ulcorneryiococcus nwem (MRSA) strams were isolated from the generd sulzely ward, but major strains of type IV were Isolated from the otorhinolq~ngology of the hospital's outpatient clinic center. All of the 88 st~nins were sensitive to vancomycin and teicoplanin, but 71 (81%) strains showed multi-drug resitant to penicillin, cephalotl~n, eiythroinycin, gentan~ycin, imipenem, clindamycin, ciprofloxacin and ooxacillin. Yo relationship was found between the antibiotic resistance pattems aud the coagulase typing patterns.


coagulase typing;mec A gene;Methicillin Resistant Staphylococcus aureus (MRSA);Staphylococcal Scalded Skin Syndrom (SSSS);Staphylococcus aureus


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