Microbial Decomposition of s-Triazine Herbicides, Atrazine and Simazine by a TNT-degrading Bacterium

TNT-분해세균에 의한 s-Triazine계 제초제인 Atrazine과 Simazine의 미생물학적 분해

  • 오계헌 (순천향대학교 자연과학대학 생명과학부) ;
  • 이명석 (순천향대학교 자연과학대학 생명과학부) ;
  • 장효원 (순천향대학교 자연과학대학 생명과학부) ;
  • 소재성 (인하대학교 생물공학과)
  • Published : 2000.09.01

Abstract

The purpose of U7is work was to iilvestigate the degradation of s-h~azine hel-hicidcs, ahilzine and simazine by TNT-degrader under several relevaut physicochemical environ~nental parameters. TNT-degrader showed effective degradability of atrazine and snnazine as well. Both atrazme (GO 1i1~11) and simazine ( 4 5 rng//) were completely degraded within 30 hrs and 4 days of incubation, respectively. As d ~ e concentrations of atrazine and sunazine increased in the media, the degradation ofthose compounds were delayed. Additional caubans were essential to degrade atrazine and simazule, and no degradation was achieved in the absence of additional carbons. The effect of supplemented nitrogens on the degradation of atrazine and sunazine was evalualed. Addition of a suppleinented nitrogen in he growth medium containing ah-azine or siinazine showed partial degr-adation olihose herbicides duriug the incubation period. However, complete degradation of atrazine and simazu~e was examined ul the absence or any supplemented nitrogens. Addltion of yeast extract in this study was inhibilory to atrazine aud siinazine degradations, respectively. TNT-degrader was a small Gram-negative cocco-bacillus. Physiological analysis using BIOLOG sysleln revealed that this strain was Ste~~ol~~opl~orno~~ns rrialtophilia.

Keywords

atrazine;biodegradation;simazine;TNT-degrader

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