Microbial Decomposition of s-Triazine Herbicides, Atrazine and Simazine by a TNT-degrading Bacterium

TNT-분해세균에 의한 s-Triazine계 제초제인 Atrazine과 Simazine의 미생물학적 분해

  • 오계헌 (순천향대학교 자연과학대학 생명과학부) ;
  • 이명석 (순천향대학교 자연과학대학 생명과학부) ;
  • 장효원 (순천향대학교 자연과학대학 생명과학부) ;
  • 소재성 (인하대학교 생물공학과)
  • Published : 2000.09.01


The purpose of U7is work was to iilvestigate the degradation of s-h~azine hel-hicidcs, ahilzine and simazine by TNT-degrader under several relevaut physicochemical environ~nental parameters. TNT-degrader showed effective degradability of atrazine and snnazine as well. Both atrazme (GO 1i1~11) and simazine ( 4 5 rng//) were completely degraded within 30 hrs and 4 days of incubation, respectively. As d ~ e concentrations of atrazine and sunazine increased in the media, the degradation ofthose compounds were delayed. Additional caubans were essential to degrade atrazine and simazule, and no degradation was achieved in the absence of additional carbons. The effect of supplemented nitrogens on the degradation of atrazine and sunazine was evalualed. Addition of a suppleinented nitrogen in he growth medium containing ah-azine or siinazine showed partial degr-adation olihose herbicides duriug the incubation period. However, complete degradation of atrazine and simazu~e was examined ul the absence or any supplemented nitrogens. Addltion of yeast extract in this study was inhibilory to atrazine aud siinazine degradations, respectively. TNT-degrader was a small Gram-negative cocco-bacillus. Physiological analysis using BIOLOG sysleln revealed that this strain was Ste~~ol~~opl~orno~~ns rrialtophilia.




  1. Appl. Environ Microbiol. v.64 Molecular basis of a bacterial consortium interspecies catabolsim of atrazine De Souza,M.L.;D.Newcombe;S.Alvey;D.E.Crawley;A.Hay;M.J.Sadowsky;L.P.Wackett
  2. Biotechnol Bioeng v.27 Biological treatment specific for an industrial wastewater containing s-triazines Hogrefe,W.;H.Grossenbacher;A.M.Cook;R.Hutter
  3. Appl. Environ, Microbiol v.64 Biodegradation of atrazine by Agrobacterium radiobacter J14a and use of this strain in bioremediation of contammated soil Struthers,J.K.;K.Jayachandran;T.B.Mooman
  4. J. Agric. Food. Chem v.45 Genes encoding s-triazine dogradation are plasmid-bome in Klebsiella pneumociae strain 99 Karns,J.S.;R.W.Eaton
  5. 농약연보
  6. Biotechnol. Lett v.18 no.9 Degradation of dimethlamine salt of 2,4-dichlorophenoxyzcetate by a mixed bacterial culture on stirred tank reactors Oh,K.H.;M.S.Cha
  7. J.Agric. FOod Chem. v.29 s-Triazines as nitrogen sources for bacteria Cook.A.M.;R.Hutter
  8. Appl. Environ Microbiol v.59 Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures Mandelbaum,R.T.;L.P.Wackett;D.L.Allan
  9. Appl. Environ Microbiol v.61 A single cytochrome P-450 system is involved in degradation of the herbicdes EPTC(s-ethyl dipropylthocarbamatic) and atrazine by Rhodococcus sp. strain NI86/21 Nagy,I.;F.Compenolle;K.Ghys;J.Vanederleyden;R.D.Mot
  10. Appl. Environ. Microbiol. v.64 Isolation and characterization of a Pseudomonas sp the miceralizes the striazine herbiede atrazine Mandebaum,R.T.;D.L.Allan;L.P.Wackett
  11. Appl. Environ. Microbiol. v.59 Metabolism of the herbicied attrazine by Rhodococcus strains. Behki,R.;E.Topp;W.Dick;P.Germon
  12. Bull. Environ Contam. Toxicol v.55 Biodegradation of the phenoxy herbicide MCPA by microbial consorta isolated from a rice field Oh,K.H.;S.K.Ahn;K.H.Yoon;Y.S.Kim
  13. J. Chromatogy v.48 2-Chloro-amino-1,3,5-triazine-6(5H)-one: a new intermediate in the biodegiadation of chlorinated s-triazines Grossenbacher,H.;C.Hom;A.M.Cook;R.Hutter
  14. Appl. Environ. Microbiol v.61 Degradation and minerahzation of atrazine by a soil bacterial isolate Radoservich,M.;S.J.Traina;Y.L.Hao;O.H.Tuovmen
  15. Appl. Environ. Microbiol v.62 Effects of atrazine on Ochrobactrium anthropo membrane fatty acids Laurn,D.;G.De Socio;R.Frassanto;D.Rotilio
  16. J. Agirc. Food. Chem v.13 Microbial degradation of simazine Kaufinan,D.D.;P.C.Kearney;T.J.Sheers
  17. J. Agric. Food Chem. v.13 Metabolites of simazinc by Aspergillus fumigatus Kearney,P.C.;D.D.Kaufman;T.J.Sheers
  18. Appl. Environ. Microbiol v.60 Purification and characterization of an inducible s-triazine hydrolase from Rhodococcus corallumus NRRL B1544R Mulbry,W.W.
  19. Environmental Ecology, 2nd ed. Freedman,B.
  20. North Carolina State University database Water resource characterzation, DSS-Pesticides/Herbicides North Carolina State University
  21. Kor. J. Biotechnol. Bioeng. v.14 Effect of various physiochemical factors on the biodegradation of explore 2,4,6-tronitrotolune by Stenotrophomonas maltophata Kim,Y.J.;M.S.Lee;Y.S.Cho;H.K.Han;S.Kim;K.H.Oh