Cloning of a Laccase Gene Fragment from Ganoderma lucidum

영지버섯 Laccase 유전자의 구리결합부위 I과 IV사이 지역의 클로닝

  • 조지현 (강원대학교 자연과학대학 생명공학부) ;
  • 최형태 (강원대학교 자연과학대학 생명공학부) ;
  • 김경훈 (강원대학교 자연과학대학 생명공학부)
  • Published : 2000.09.01


Degenerate primevs corresponding to the consensus sequences of the copper-binding regions in the N- and Cterminal domains of fungal laccases were used to isolate laccase gene-specific sequences froin a white rot rungus Ganodern~a lucidrm w-hich has been known to strengthen the imnnne system. A 1.6 Kbp fragment was amplified by PCR and its base sequence was detenuiued. Locating seven iutrous within the base sequence, we could deduce its amino acid sequence. The nucleotide sequence witl~out introlls was 47Y0 identical to that of lee1 gene of Pametes wllosa; lhe identity in amino acid sequences of the two was 7994 The deduced amino acid seqoence was also sunilar to those of Coriolus versicolo~ kc3 (79%); Co~,iolz~s hirsutus phenolouiduse (78%), Trainetes vel.srcoloi. lccl (77%), Trametes ~!i/Iosa Ice2 (77%) and Trametes vemicolor kc4 (66%).


Ganoderma lucidum;laccase;PCR;white-rot fungi


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