Cloning of a Laccase Gene Fragment from Ganoderma lucidum

영지버섯 Laccase 유전자의 구리결합부위 I과 IV사이 지역의 클로닝

  • 조지현 (강원대학교 자연과학대학 생명공학부) ;
  • 최형태 (강원대학교 자연과학대학 생명공학부) ;
  • 김경훈 (강원대학교 자연과학대학 생명공학부)
  • Published : 2000.09.01

Abstract

Degenerate primevs corresponding to the consensus sequences of the copper-binding regions in the N- and Cterminal domains of fungal laccases were used to isolate laccase gene-specific sequences froin a white rot rungus Ganodern~a lucidrm w-hich has been known to strengthen the imnnne system. A 1.6 Kbp fragment was amplified by PCR and its base sequence was detenuiued. Locating seven iutrous within the base sequence, we could deduce its amino acid sequence. The nucleotide sequence witl~out introlls was 47Y0 identical to that of lee1 gene of Pametes wllosa; lhe identity in amino acid sequences of the two was 7994 The deduced amino acid seqoence was also sunilar to those of Coriolus versicolo~ kc3 (79%); Co~,iolz~s hirsutus phenolouiduse (78%), Trainetes vel.srcoloi. lccl (77%), Trametes ~!i/Iosa Ice2 (77%) and Trametes vemicolor kc4 (66%).

Keywords

Ganoderma lucidum;laccase;PCR;white-rot fungi

References

  1. J. Biol. Chem. v.265 Cloning, sequence analysis, and expression of liginolytic phenoloxidase genes of the white-rot basidiomycete Coriolus nirsutus Kojima,Y.;Y.Tsukuda;Y.Kawan;A.Tsukamoto;J.Sugiura;M.Sakaino;Y.Kita
  2. J. Mol. Biol. v.224 Refined crystal structure of ascorbato oxidase at 1.9Å resolution Messerschmidt,A.;R.Ladcnstem;R.Huber;M.Bolognesi;L.Avighno;R.Petruzzelh;A.Rossi;A.finazzi-Agro
  3. Nucl. Acids. Res. v.20 A simple and efficient protocol for isolation of high molicular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues Moller,E.M.;G.Bahnweg;H.Sandermann;H.H.Geiger
  4. FEMS Micorbiol Lett v.155 Cloning and sequencing of a second laccase gene from the white-rot fungus Coriolus versicolor Mikuni,J.;N.Morohoshi
  5. Appl. Envrion Microbiol v.62 Isolation of laccase gene-specific sequences from white rot and brown rot fungi by PCR D'Souza,T.M.;K.Boomnathan;C.A.Reddy
  6. Cloning and Characterization of three laccase genes from the white-rot basidiomycete Trametes villosa: genomic organization of the laccase gene family gene v.181 Yaver,D.S.;E.J.Gohghtly
  7. Appl. Envrion. Microbiol v.59 Characterization and structural analysis of the laccase Ⅰgene from the newly isolated ligninolytic basidomycete PMI(CECT 2971) Coll,P.M.;C.Tabemero;R.Santamana;P.Perez
  8. 미생물학회지 v.33 Coprimus congregatus에서 산성액체배지에서의 laccase 역할 김순자;임영은;최형태
  9. 미생물학회지 v.29 Coprinus congregatus의 세포막 연관 laccase의 세포의 분비 김순자;최형태;강사욱;하영칠
  10. Gene v.196 Cloning and sequence analysis of two laccase comlementary DNAs from the liginolytic basidionycete Trametes versicolor Ong,E.;W.B.R.Pollock;M.Smith