Characterization of Subunits Dissociated from Cellulosome of Clostridium thermocellum JW20

Clostridium thermocellum JW20가 생성하는 섬유소분해 효소복합체(cellulosome) 구성단백질의 특성에 관한 연구

  • 최상기 (생명공학연구소 미생물공정연구실)
  • Published : 2000.09.01


The cellulosome of Clo.~tr~rlil~m tl\ulcornererfnocellum consistmg of 26 dfferent polypeptides contains calcium. The polypeptides dissociated when calcium was removed. Most of dockerill region in the catalytic polypeptides cleavcd during dmociation. The dissociated polypeptides were well separated by MonoQ column chromatography into CipA containing fraction, a fraction still complexed wit11 91 kDa (CelK-a). 60 IiDa and 57 kDa polypeptides, and fractious contailling mainly single polypeptide of 46 kDa (CelA-a) or 71 1d)a polypeptide (CelS-trj Most or the fractions hydrolyzed c~ystalliue cellulose The purified 71 kDa polypeptide was strictly dependent on calcium for crystalline cellulose hydvolyzing activities a1 $60^{\circ}C$~$70^{\circ}C$ but 46 kDa polypeptide was not. 46 M)a polypeptide digested cellodextri~~ as cellobiose or cellotriose unit, and glucose was produced together with cellobiose and cellotriose froln cellotetraosc. It seems that cellulosome produces final product, cellobiose, through coordinated ~qulation of activities of vannus subunits.




  1. Biochemistry v.35 Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethlene diaminetelraacetic acid occurs with the formation of truncated polypeptides Choi,S.K.;L.G.Ljungdahl
  2. The prokaryotes, 2nd ed. Cellulose-decomposing bacteria and their enzyme systems. i Coughlan,M.P.;F.Mayer;A.B.Balows(ed.)
  3. Proc. Natl. Acad. Sci. USA v.91 Cl-Cx revisited: Intramolecular synergism in a celluase Din,N.;H.G.Damude;N.R.Gikes;R.C.Jr.Miller;R.A.Warren;D.J.Kiburn
  4. Proc. Natl. Acad. Sci. USA v.92 The anchorage function of CipA(Cell).a scaffolding protein of the Clostridum thermocellum cellulosome Kruus,K.;A.C.Lua;A.L.Demain;J.H.D.Wu
  5. Methods Enzymol v.160 Macromolecular complexes and yellow affinity substance from Clostridum thermocellum Ljungdahl,L.G.;M.P.Coughlan;F.Mayer;Y.Mori;K.Honami
  6. Appl. Environ. Microbiol. v.53 Macromolecular organization of the cellulolytic enzyme complex of Clostridium thermocellum as revealed by electron microscopy Mayer,F.;M.P.Coughlan;Y.Mori;L.G.Ljungdahl
  7. FEMS Microbiol. Lett. v.94 Cloning of a Clostridium thermocellum DNA fragment encoding polypeptides that bind the catalytic components of the cellulosome Fujino,T.;P.Beguin;J.P.Aubert
  8. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmh,U.K.
  9. FEMS Microbiol. Lett. v.51 A catalogue of Clostridum thermocellum endoglucanase. β-glucosidase and xylanase genes cloned in Eschericha coli Hazlewood,G.P.;M.P.N.Romamee;K.Davidson;O.Grepinet;P.Beguin;J.Millet;O.Raynaud;J.P.Aubert
  10. Biochem. J. v.121 The cellulase of Fusarium solani Purification and specificity of the β-1,4-D-glucanase and β-glucosidase components Wood,T.M.
  11. J. Bacteriol. v.156 Adherence of Clostridum thermocellum to cellulose Bayet,E.A.;R.Kenig;R.Lamed
  12. J. Bacteriol. v.177 Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component Kruus,K.;W.K.Wang;J.Ching;J.H.D.Wu
  13. Biochemistry v.27 Two components of an extracellular protein aggregate of Clostridium thermocellum together degrade crystalline cellulose Wu,J.H.D.;W.H.D.Johnson;A.L.Demain
  14. Mol. Microbiol. v.8 Sequencing of a Clostridum thermocellum gene(cipA)encoding the cellulosomal SI protein reveals an unusual degree of internal homology Gergross,V.T.;P.M.Romaniec;N.S.Huskisson;A.L.Deman
  15. J. Biochem. Biophys. Meth. v.11 Determination of reducing sugars in the nanomole range with tetrazolum blue Jue,C.K.;P.N.Lipke
  16. Microbial enzymes and biotechnology Cellulose degradation by fungi Coughlam,M.P.;W.M.Fogarty(ed.);C,T,Kelly(ed.)
  17. Ann. Rev. Microbiol. v.47 The cellulosome: the exocellular organelle of Clostridum Felix,C.R.;L.G.Ljungdahl
  18. J. Bacteriol. v.162 Sequence of celluase gene of the thermophilic bacterium Clostridum thermocellum Beguin,P.;P.Cornet;J.P.Aubert
  19. Appl. Environ. Microbiol. v.56 Subunit composition and glycoside activities of the cellulase complex from Clostridium thermocellum JW20 Kohrung,S.;J.Wiegel;F.Mayer
  20. Lipids v.1 Chromatographic and electrophoertic technigucs Lake,B.D.;H.J.Goodwin;I.Smith(ed.);J.W.T.Seakins(ed.)
  21. Appl. Environ. Microbiol. v.51 Properties of a Clostridium thermocellum endoglucanase produced in Eschericha coli Schwarz,W.H.;F.Grebrutz;W.L.Staudenbaucr
  22. J. Bacteriol v.173 Isolation and properties of a major cellobiohydrolase from the celluosome of Clostridum thermocellum Morag,E.;I.Halevy;E.A.Bayer;R.Lamed
  23. Biochemistry v.35 Structural role of calcium for the organization of the cellulosome of Clostridium thermocellum Choi,S.K.;L.G.Ljungdahl
  24. J. Bacterol v.181 Cloning and sequence analysis of a new cellulase gene encoding CelK, a majo cellulosome component of Clostridium thermocellum evidence for gene duplication and recombination Kataeva,I.;X.L.Li;H.Chen;S.K.Choi;L.G.Ljungdahl
  25. FEBS Lett. v.291 Interaction of the duplicated segment carried by Clostridum thermocellum cellulase with cellulosome components Tokathds,K.;S.Salamitou;S.P.Beguin;P.Dhurjati;J.P.Aubert
  26. Biochimie v.63 Purification and properties of an endo-β-1,4-glucanase from Clostridium thermocellum Petre,J.;R.Longin;J.Millet
  27. J. Bacteriol v.171 Cloning and DNA sequencing of the gene coding for Clostridium thermocellun cellulase Ss(CelS), a major cellulosome component Wang,W.K.;K.Kruus;J.H.D.Wu