Analysis of 16S-23S rRNA Intergenic Spacer Regions of Aeromonas veronii biogroup sobria and A. caviae

Aeromonas veronii biogroup sobria와 Aeromonas caviae의 16S-23S rRNA Intergenic Spacer Regions 분석

  • 강동율 (부경대학교 미생물학과 병원 미생물실) ;
  • 이훈구 (부경대학교 미생물학과 병원 미생물실)
  • Published : 2000.09.01

Abstract

The intern1 spacer regions (ISR) between the 16s and 23s $1_RNA$ genes of Aeronzonus iwonii blogroupsobria and A. caviae were investigated by PCR fragment length typing and DNA sequencing. A. iwonii bv.sobria has a speciIic 16s-23s pattern of 2-4 fiagments ranging Goin 479-539 bp, with the exception of thespecies Aeron7onns cmiae, which has 3 fragments ranglog from 470-602 bp. In all of the.4 vei*onii bv. sobr,iaand A, caviae strains examined in this study, the 470-481bp Tragnent, designated TSR-1, invariably contained $tDNA^{uc(GAT)$ and $tDNA^{Ala(TGC)$ in contrast to ISR-2 (513-525 bp). ISR-3 (537-539 bp) and ISR-4 (568-602 bp)containing TEX>$tDNA^{Olu(ITC)$ A stretch of 20 nucleotides (178-197 bp) in the ISR-4 was conserved only wit11mA.caiiue, from which the A. caiiae specific primer, named prAC-F, was designed and used for PCR with aAcaviae coimnon reverse primer A PCR product of 450 bp was apparent alnong I , caiizne strains, but not ii1.4.ijeronii bv. sob~ia strains. The PCR product was oot detected t"-om strains belonging to A. hjili-o~~hila, Ebrio,aud the family Ef\ulcornertei,obncteriaceae. This study provides the first molecular tool for mdentifying the species 8.caviae.ing the species 8. caviae.

Keywords

Aeromonas veronii bv. sobria;Aeromonas caviae;16S-23S rRNA intergenic spacer region;ISR-PCR;tRNA;species-specific primer

References

  1. Appl. Environ. Microbiol. v.61 Comparative analysis of the 16S to 23S ribosomal intergenic spacer sequences of Bacillus thuringiensis strains and subspecies and of closely related species Bourque,S.N.;J.R.Valero;M.C.Lavoie;R.C.Levesque
  2. Appl. Environ. Microbiol. v.65 Analysis of 16S-23SrRNA Intergenic Spacer Regions of Vibrio cholerae and Vibrio mimicus Chun,J.;A.Huq;R.C.Rita
  3. Int. J. Syst. Bacteriol. v.42 International Committee on Systematic Bacteriology Subcommitte on the taxonomy of Vibrionaceae 1992-00-00
  4. Int. J. Syst. Bacteriol. v.42 rRNA gene restriction patterms as taxonomic tools for the genus Aeromonas Lucchini,M.G.;M.Altwegg
  5. J. Bacteriol. v.180 Variation of the ribosomal operon 16S-23S gene spacer region in representatives of Salmonella enterica subspecies Luz,S.P.;F.Rodriguez-Valera;R.Lan;P.R.Reeves
  6. J. Mol. Biol. v.3 A Procedure for isolation of deoxyribonucleic acid from microorganisms Marmur,J.
  7. 감염. v.19 환자와 자연환경에서 분리된 Aeromonas의 균종 및 항균제 감수성 곽창길;정윤섭;이삼열
  8. 대한미생물학회지 v.34 담수어 양식장 및 해수로부터 Aeromonas屬의 동정 및 항균제 내성에 관한 연구 강동률;이훈구
  9. FEMS Microbiol Lett. v.106 Molecular characterization of DNA encoding 23S rRNA and 16S-23S rRNA neergenic spacer regions of Aeromonas hydrophila East.A.K.;M.D.Collins
  10. J. Clin Microbiol v.28 Microbiologic and clinical evidence supporting the role of Aeromanas cavae as a pediatric enteric pathogen Namdari,H.;J.B.Edward
  11. Microbiology v.143 Identification of staphylococcal and streptococcal causes of bovinomastitis using 16S-23SrRNA spacer regions Forsman,P.A.;Tilsala-Timisjarvi;T.Alatossava
  12. Can. J. Microbiol. v.40 Characterization of Aeromonas genomic species by using quinone, polyamine, and fatty acid patterns Kampfer,P.;K.Blasczyk;G.Auling
  13. J. Clin. Microbiol v.29 Aeromonas trota sp nov. and ampicillin-susceptible species isolated from clinical speecimens Carnahan,A.M.;T.Chakraborty;G.R.Fanning;D.Verma;A.Ali;J.M.Janda;S.W.Joseph
  14. Microbiology v.141 Typing of Staphylococcus aureus strains by PCR amplification of variable-length 16S-23SrDNA spacer regions: characterization of spacer sequences Gurtler,V.;B.Helen
  15. Microbiology v.145 Diffcrentiation of Campylobacter coh and C.jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region Christensen,H.;K.Jorgensen;E.O.John
  16. Clin Infect. Dis. v.27 Evolving concepts regarding the genus Aerominas, an expanding panorama of species, diesease presentations, and unanswered questions Michael J.;L.A.Sharon
  17. Int. J. Syst. Bacteriol. v.43 Aeromonas enteropelegenes and Aeromonas ichihiosmia are identical to Aeromoas trota and Aeromonas veronin,respectively, as revealed by small-subunit rRNA sequence analysis. Collins,M.D.;A.J.Martinez-Murcia;J.Cai
  18. J. Clin. Microbiol. v.35 Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16SrRNA genes Borrell,N.;S.G.Acinas;M.J.Figueras;A.J.Martinez-Murcia
  19. 한국미생물학회지 v.30 양식 가물치 궤양병의 병리 연구 이훈구
  20. Gene v.96 High efficiency transformation of Escherichia coli with plasmids Inoue,H.;H.Nojima;H.Okayama
  21. J. Bacterol. v.178 Comparison of the small 16S to 23S Intergenic Spacer Region(ISR) of the rRNA operons of some Eschericha coli Strains of the ECOR collection and E coli K-12 Garcia-Martnez,J.A.;Martivez-Murcia;A.L.Anton;R.V.Francisco
  22. Microbiology v.142 New approaches to typing and identification of bacteria using the 16S-23SrDNA spaccr region Gurtler,V.;A.S.Vilma
  23. Int. J. Syst. Bacteriol. v.46 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter and intraspecific Bifodobacterium Phylogeny Leblond-Bourget,N.H.Philippe;I.Mangin;D.Bernard
  24. J. Appl. Bacteriol. v.77 Rapid identification of Aeromonas species using 16SrDNA targeted ohgonucleotide primers: a molecular approach based on screening of environmental isolates Dorsch,M.;N.J.Ashbot;P.T.Cox;A.E.Goodman
  25. J. Clin. Microbiol. v.29 Aerokey Ⅱ: A flexible key for identifying clinical Aeromonas species Carmahan,A.M.;S.Behram;S.W.Joseph
  26. J. Bacteriol. v.174 Acholeplasma Iaidlawi has tRNA genes in the 16S-23S spacer of the rRNA operon Nakagawa,T.;T.Uermori;K.Asada;I.Kato;H.Ryo
  27. Int. J. Syst. Bacteriol. v.44 Cellular fatty acid composition as a chemotaxonomic marker for the differentiation of phenospecies and hybridization groups in the genus Aeromonas Huys,G.;M.Vancanneyl;R.Coopman;P.Janssen;E.Falsen;M.Altwegg;K.Kersters