Discrimination of Irradiated Beef Using Comet Assay

Comet assay를 이용한 방사선 조사육의 판별

  • Jeong, Seok-Kyu (Institute of Functional Foods and Safety, Handong University) ;
  • Park, Jong-Heum (Institute of Functional Foods and Safety, Handong University) ;
  • Ji, Seung-Taek (Institute of Functional Foods and Safety, Handong University) ;
  • Park, Kum-Ju (Institute of Functional Foods and Safety, Handong University) ;
  • Kim, Hai-Hong (Institute of Functional Foods and Safety, Handong University) ;
  • Hyun, Chang-Kee (School of Bioscience and Food Technology, Handong University) ;
  • Shin, Heuyn-Kil (School of Bioscience and Food Technology, Handong University)
  • 정석규 (한동대학교 기능성식품 및 안전성 연구소) ;
  • 박종흠 (한동대학교 기능성식품 및 안전성 연구소) ;
  • 지승택 (한동대학교 기능성식품 및 안전성 연구소) ;
  • 박금주 (한동대학교 기능성식품 및 안전성 연구소) ;
  • 김해홍 (한동대학교 기능성식품 및 안전성 연구소) ;
  • 현창기 (한동대학교 생물식품공학부) ;
  • 신현길 (한동대학교 생물식품공학부)
  • Published : 2000.08.30

Abstract

DNA damages in post-mortem bovine muscle samples caused by gamma irradiation at doses of 1 to 10 kGy were determined by Comet assay. When the cell extract was prepared in a physical method and followed by neutral lysis and neutral electrophoresis, the optimal comet images could be obtained. DNA damages were evaluated from the mean tail length, the distributions of comet images in 4 groups divided by tail length and the relative damage index (RDI) values calculated from the distribution pattern. The mean tail length and RDI value were increased by increasing the irradiation dose, and the RDI value was found to be useful as an index for discriminating of irradiation and measuring the irradiated dose. Blind tests using Korean domestic (Hanwoo) and imported beef samples showed a higher RDI value for the latter. However, the value was lower than those of irradiated samples indicating that the cause of DNA damages in the imported beef samples might be not irradiation but low-temperature treatments. It was concluded from the results of this study that the irradiated beef and its irradiated dose could be detected and predicted by Comet assay.