Synergistic Effects of Natural Medicinal Plant Extracts on Growth Inhibition of Carcinoma (KB) Cells under Oxidative Stress

산화적 상해로 인한 상피세포암 세포(KB) 억제에 미치는 천연약용식물 추출물의 상승효과

  • Kim, Jeong-Hee (Department of Oral Biochemistry, College of Dentistry, Institute of Oral Biology, College of Dentistry, KyungHee University) ;
  • Ju, Eun-Mi (Department of Oral Biochemistry, College of Dentistry, Institute of Oral Biology, College of Dentistry, KyungHee University) ;
  • Kim, Jin-Kyu (Korea Atomic Energy Research Institute)
  • 김정희 (경희대학교 치과대학 구강생화학교실) ;
  • 주은미 (경희대학교 치과대학 구강생화학교실) ;
  • 김진규 (한국원자력연구소)
  • Published : 2000.12.30

Abstract

Medicinal plants with synergistic effects on growth inhibition of canter cells under oxidative stress were screened in this study. Methanol extracts from 51 natural medicinal plants which were reported to have anticancer effort on hepatoma stomach cancer or colon cancers which are frequently found in Korean, were prepared and screened for their synergistic activity on growth inhibition of cancer cells under chemically-induced oxidative stress by using MTT assay. Twenty seven samples showed synergistic activity on the growth inhibition in various extent under chemically-induced oxidative stress. Among those samples, eleven samples such as Melia azedarach, Agastache rugosa, Catalpa ovata, Prunus persica, Sinomenium acutum Pulsatilla koreana, Oldenlandia diffusa, Anthriscus sylvestris, Schizandra chinensis, Gieditsia sinensis, Cridium officinale, showed decrease in $IC_{50}$ values more than 50%, other 16 samples showed decrease in $IC_{50}$ values between 50-25%, compared with the value acquired when medicinal plant sample was used alone. Among those 11 samples, extract of Catalpa ovata showed the highest activity. $IC_{50}$ values were decrease to 61% and 28% when carcinoma cells were treated with Catalpa ovata extract in combination of 75 and 100 uM of hydrogen peroxide, respectively.