Comparison of TNF-Mediated Glucose Catabolism between the TNF-Sensitive and -Resistant Cell Lines

  • Kim, Yeon-Hyang (Department of Biochemistry, College of Science and Bioproducts Research Center, Yonsei University) ;
  • Park, Bok-Ryun (Department of Biochemistry, College of Science and Bioproducts Research Center, Yonsei University) ;
  • Cheong, Hee-Sun (Department of Biochemistry, College of Science and Bioproducts Research Center, Yonsei University) ;
  • Kwon, Oh-Hwan (Department of Biochemistry, College of Science and Bioproducts Research Center, Yonsei University) ;
  • Kim, Dae-Que (Department of Biochemistry, College of Science and Bioproducts Research Center, Yonsei University) ;
  • Kim, Soung-Soo (Department of Biochemistry, College of Science and Bioproducts Research Center, Yonsei University)
  • Received : 1998.09.29
  • Accepted : 1998.11.24
  • Published : 1999.03.31

Abstract

When murine fibrosarcoma L929 cells, a TNF-sensitive cell line, were treated with recombinant human tumor necrosis factor-$\alpha$ (rhTNF-$\alpha$), the activities of glycolytic regulatory enzymes and lactate dehydrogenase increased up to 100-150% compared to the control L929 cells after TNF treatment. By using various metabolic inhibitors and activators, it was found that cAMP-dependent protein kinase is responsible for the increase of activities of the glycolytic enzymes. The activities of glycolytic regulatory enzymes and lactate dehydrogenase of TNF-resistant A549 cells, a human lung carcinoma cell line, did not increase significantly compared to TNF-sensitive L929 cells upon TNF treatment. In contrast, the pyruvate carboxylase activities of A549 cells, but not L929 cells, increased up to 30~40% after TNF treatment. The data suggest that pyruvate carboxylase activity may contribute to the compensation of energy loss mediated by TNF treatment in TNF-resistant A549 cells.

Keywords

cAMP;Glucose uptake;Glycolytic enzyme;Lactate dehydrogenase;Pyruvate carboxylase;Tumor necrosis factor

Acknowledgement

Supported by : Bioproducts Research Center at Yonsei University, Korea Science and Engineering Foundation (KOSEF)