Synthesis of Partially Hydrolyzed Water-soluble Chitosans and their Antibacterial Activities

부분 가수분해된 수용성 키토산의 제조와 그 항균성에 관한 연구

  • 김천호 (한국과학기술연구원 생체재료연구센터) ;
  • 이응석 (중앙대학교 생물공학과) ;
  • 함영태 (중앙대학교 생물공학과) ;
  • 김병용 (경희대학교 식품가공학과) ;
  • 손태일 (중앙대학교 생물공학과)
  • Received : 1997.12.22
  • Accepted : 1998.11.02
  • Published : 1999.02.10

Abstract

Chitosan was partially degraded by using nitrous acid. The deacetylation degree of chitosan decreased with its degree of hydrolysis. Deacetylation degree of each fraction was less than 50%. The degraded product was fractionated by means of precipitation using aqueous methanol solution or aqueous methanol-acetone solution. The molecular weight of each fraction was distributed between 6000 and 4000, and below 2000 for being precipitated using aqueous methanol solution and aqueous methanol-acetone solution respectively. They had narrow molecular weight distributions, and their polydispersities were less than 1.7. The antibacterial activities for each fraction were evaluated against Bacillus subtilis HB 101 and E. coli PP 2, gram-positive bacteria and gram-negative bacteria, respectively. Fraction B (Mw=5100) showed high antibacteiral activity. All fractions were more active against Bacillus subtilis than E. coli.

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