Measurement of Langmuir Adsorption Equilibrium by Elution-curve Method and Frontal Analysis

용출곡선법과 Frontal Analysis를 이용한 Langmuir 흡착평형식의 측정

  • Choi, Yong Seok (Department of Chemical Engineering, Inha University) ;
  • Lee, Chong Ho (Department of Chemical Engineering, Inha University) ;
  • Row, Kyung Ho (Department of Chemical Engineering, Inha University)
  • Received : 1998.11.30
  • Accepted : 1999.02.06
  • Published : 1999.08.10

Abstract

Adsorption isotherm is the most fundamental information in adsorption separation-process. Directly from the elution profile of a peak, the elution-curve method and frontal analysis(FA) were utilized to measure the adsorption isotherm in this work. Using RP-HPLC, sample and the buffer added in mobile phase were 5'-GMP and sodium phosphate, respectively. In this experimental condition, the retention time was decreased with increase in the injected mass of sample. And the front part of a peak was very stiff, so Langmuir adsorption isotherm might be applied. By the elution-curve method, the parameters used in the isotherm were obtained by optimization method, while by the FA, the concentrations of stationary phase were measured from the elution curve and the isotherm was determined by regression analysis. Compared to FA, the consumption of sample was less, and only one or two injections were needed by the elution-curve method. Finally, the effect of concentration of sodium phosphate in mobile phase on the parameters of the isotherm was investigated.

Acknowledgement

Supported by : 인하대학교

References

  1. 공업화학 v.9 최용석;이종호;노경호
  2. Fundamentals of Preparative and Nonlinear Chromatography G. Guiochon;S. Golshan-Shirazi;A. Katti
  3. HPLC in Biotechnology R. A. Barford;W. S. Hancock(Ed.)
  4. J. Amer. Chem. Soc. v.65 D. Devault
  5. Chromatographia v.20 Y. A. Eltekov;Y. V. Kazakevich;A. V. Kiselev;A. A. Zhuchkov
  6. J. Chromatogr. v.365 Y. A. Eltekov;Y. V. Kazakevich
  7. J. Chromatogr. v.659 H. Guan;B. J. Stanley;G. Guiochon
  8. Anal. Chem. v.34 C. N. Reilly;G. P. Hildebrand;J. W. Ashley Jr.
  9. Anal. Chem. v.61 S. Golshan-Shirazi;G. Guichon
  10. Anal. Chem. v.63 E. V. Dose;S. Jacobson;G. Guiochon
  11. J. Chromatogr. v.193 A. W. J. de Jong;J. C. Kraak;H. Poppe;F. Nooitgedacht
  12. 공업화학 v.8 이주원;노경호
  13. J. Chem. Eng. Data v.41 A. Akgerman;M. Zardkoohi
  14. J. Chem. Eng. Japan v.19 K. H. Row;W. K. Lee