Asian-Australasian Journal of Animal Sciences (아세아태평양축산학회지)
- Volume 12 Issue 5
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- Pages.695-701
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- 1999
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- 1011-2367(pISSN)
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- 1976-5517(eISSN)
DOI QR Code
Gene Expression of Arginine Vasotocin in Ovarian and Uterine Tissues of the Chicken
- Saito, N. (Laboratory of Animal Physiology, Graduate School of Bioagricultural Science, Nagoya University) ;
- Grossmann, R. (Institute for Small Animal Research)
- Received : 1998.12.08
- Accepted : 1999.01.14
- Published : 1999.08.01
Abstract
The hypothalamus is the classic site of synthesis of arginine vasotocin as neurohypophyseal hormone in the chicken. However, high concentrations of arginine vasotocin were also measured in ovarian tissues by radioimmunoassay. At first, we observed specific positive signal of mRNA encoding AVT in the hypothalamus by Northern hybridization. However, we could not find any specific bands in ovarian and uterine tissues. For evidence of transcription of the arginine vasotocin gene ingonadal tissues of the chicken, this study has applied the polymerase chain reaction as a highly sensitive assay. The hypothalamus, the four largest preovulatory ovarian follicles and the shell gland (uterus) were collected at 4 h and 20 h before oviposition. The ovarian follicular tissues were separated into granulose theca interns and theca externa layers. The uterine tissues were separated into myometrium and endometrium The extracted mRNA was converted to cDNA by reverse-transcriptase using oligo-
Keywords
Chicken;Ovary;Uterus;Arginine Vasotocin;RT-PCR