Some Cultural Characteristics of Bacillus megaterium YWO-5 Producing Bioflocculant for Wastewater Treatment

폐수처리용 생물응집제를 생산하는 Bacillus megaterium YWO-5의 배양특성

  • Seo, Ho-Chan (Institute of Biotechnology, Korea University) ;
  • Yeo, Sung-Jee (Graduate School of Biotechnology, Korea University) ;
  • Cho, Hong-Yeon (Graduate School of Biotechnology, Korea University) ;
  • Yang, Han-Chul (Graduate School of Biotechnology, Korea University)
  • 서호찬 (고려대학교 생명공학연구소) ;
  • 여승재 (고려대학교 생명공학원) ;
  • 조홍연 (고려대학교 생명공학원) ;
  • 양한철 (고려대학교 생명공학원)
  • Published : 1999.02.01


To develop bioflocculant for wastewater treatment, about 60 type culture strains and 450 strains isolated from natural sources were examined for screening their ability to flocculate the swine wastewater. Among them, YWO-5 showed the highest activity for NTU removal efficiency and was identified as Bacillus megaterium according to the cultural, morphological and physiological properties. The maximum production of the flocculant was achieved in culture medium containing 2% glucose, 0.05% soytone, 0.01% $CaCl_2$, 0.05% $KH_2PO_4$, and 0.05% yeast extract with initial pH 6.5 when cultured with rotary shaker controlled at 20$^{\circ}C$ and 150 rpm. With jar fermentor, the maximum production was reached to NTU removal efficiency of 93% after 3 days under the optimal conditions. The bioflocculant produced by Bacillus megaterium YWO-5 was effective on various suspended solids and organic wastewaters.


Bacillus megaterium;bioflocculant;wastewater treatment