Compositional Analysis and Some Properties of Biosurfactant from Pseudomonas sp. SW1

Pseudomonas sp. Sw1이 생산하는 Biosurfactant의 조성 및 특성

  • Suk, Wan-Su (Department of Microbiology, Pusan National University) ;
  • Lim, Eun-Gyoung (Department of Microbiology, Pusan National University) ;
  • Son, Hong-Joo (Department of Biotechnology, Miryang National University) ;
  • Lee, Geon (Department of Microbiology, Pusan National University) ;
  • Lee, Sang-Joon (Department of Microbiology, Pusan National University)
  • 석완수 (부산대학교 미생물학과) ;
  • 임은경 (부산대학교 미생물학과) ;
  • 손홍주 (밀양대학교 생물공학과) ;
  • 이건 (부산대학교 미생물학과) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 1999.02.01

Abstract

A tentative composition and some properties of biosurfactants, type I and type II, from Pseudomonas sp. SW1 are described. Biosurfactant type I and II are soluble in water, dichloromethane, chloroform, and a mixture of chloroform and methanol, respectively. The UV absorption spectrum of biosurfactants showed three characteristic peaks in the range of 212, 250 and 365nm, respectively. As a result of IR spectroscopy, GC/MS analysis and biochemical analysis, biosurfactant type I was a polymeric biosurfactant containing carbohydrate, lipid and protein. The carbohydrate was characterized as rhamnose. The lipid part consists of $C_{14}-C_{23}$ fatty acid when analyzed by GC/MS. The biosurfactant type II was a rhamnolipid consisting of carbohydrate and lipid.

Keywords

biosurfactant;composition;Pseudomonas sp. SW1;IR;GC/MS