Production of Alternan by Leuconostoc mesenteroides CBI-110

Leuconostoc mesenteroides CBI-110에 의한 Alternan의 생산

  • 정호권 ((주)참존 부설 생물소재연구소) ;
  • 김광년 ((주)참존 부설 생물소재연구소) ;
  • 이홍석 (식품위생연구원 식품위생연구부) ;
  • 정선호 (건국대학교 미생물공학과)
  • Published : 1999.02.01

Abstract

Alternan known as a kind of dextran is a linear and cyclic polysaccharide which is synthesized with sucrose by alternaansucrase (EC2.4.1.140) of Leuconostoc mesenteroides. But this polysaccharide is different from dextran in its structure and other properties. For the purpose of applying alternan for the food, cosmetic, and pharmaceutical industries, we isolated an excellent alternan-porducing strain, Leu. mesenteroides CBI-110. The optimum culture conditions were studied to improve the yield, and the activity of alternansucrase was increased up to 33U/ml in the optimum culture media. The concentrations of $Mn^{2+}$ and $Cu^{2+}$ ions were 0.01% and 0.03%, respectively, for the effective production of alternan. Finally, we obtained 25.6g/L of alternan.

Keywords

Leuconostoc mesenteroides;alternansucrase;alternan