A Study on the Remaining Concentration of Pesticides in Tap Water of Taejon City by Ellman′s Enzyme Method and the Countermeasure

Ellman 효소법에 의한 대전시 상수도내 살충제의 잔류농도 결정 및 그 대책에 관한 연구

  • 이봉호 (대전산업대학교 공업화학과) ;
  • 이영순 (서울산업대학교 안전공학과) ;
  • 전종한 (대전산업대학교 공업화학과)
  • Published : 1999.02.01

Abstract

The degree of pesticides accumulation in tap water in Taejon from June 1995 to Apr 1996 was measured by Ellman's coupled enzyme assay. Since organic phosphate and carbamate pesticides specifically inhibit the neurotransmitter modulating enzyme acetylcholinesterase(AChE), the enzyme activity can be used as a diagnosis for the pesticides accumulation in water and various samples. During the period of this study, the enzyme activity was changed almost every week. The lowest enzyme activity was 64 % of that of the control reaction and there are several days showing about 100 % enzyme activity. In general, the enzyme activity is higher in summer than other seasons especially early spring times. The pH value of tap water was very close to neutral(pH 7.0) and it seems that the enzyme activity was not affected by the small pH changes. Either boiling of tap water or addition of NaOH solution decomposed the pesticide components. These results show that AChE assay is a convenient, sensitive, and reliable method for detection of pesticides in water samples.

Keywords

Tap Water;Pesticides;Remaining Concentration of Pesticides;Ellman′s Enzyme Assay;Acetylcholinesterase Inhibition

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