Effects of Natural Extracts on the Radiation-induced Pink Mutations in Tradescantia Stamen Hair Cells

  • Published : 1999.10.01


The effect of a water-soluble extract from natural materials on radiation-induced mutations was studied by means of TSH assay in Tradescantia 4430 stamen hair cells. Inflorescence cuttings, with or without pretreatments of natural extracts for 3 hours, were exposed to 1 Gy of gamma ray. Comparisons were made on the basis of pooled data during the peak interval between the mean pink mutation frequencies of the experimental groups. Pretreatments of FB or FB-I resulted in about two-fold increases of the pink mutation frequencies, compared to those of the control group. Synergism between certain fractions and radiation was a possible cause of the increased DNA damage. FB and FB-I had a radiosensitizing effects on the pink mutations in Tradescantia 4430 stamen hair cells (p<0.001). On the other hand, the extract PP in a proper concentration significantly reduced the pink mutation frequencies (p<0.05). The result means that PP has a protective effect on the radiation-induced cell damage.


  1. KAERI/RR-1903/98 Radiation Application for Development of Bioscience; Development of Enhanced Radioprotectors J.K.Kim;K.B.Park;Y.I.Lee;K.J.Chun;Y.K.Lee;K.H.Hong;D.C.Jun;C.J.Lee;W.R.Kim;H.S.Shin;T.W.Park
  2. Mut. Res. v.38 Comparative effects of ionizing radiation and two gaseous chemical mutagens on somatic mutation induction in one mutable and two nonmutable clones of Tradescantia C.H.Nauman;A.H.Sparrow;L.A.Schairer
  3. Science v.176 Mutations induced in Tradescantia by small doses of x-rays and neutrons: Analysis of dose-response curves A.H.Sparrow;A.G.Underbrink;H.H.Rossi
  4. Chemical Mutagens: Principles and Methods for their Detection v.3 A.G.Underbrink;L.A.Schairer;A.H.Sparrow;A.Hollaender(ed.)
  5. Kor. J. Environ. Biol. v.16 Effects of diurnal temperature difference and gamma radiation on the frequency of somatic cell mutations in the stamen hairs of Tradescantia 4430 J.K.Kim;W.R.Kim;J.S.Kim;H.S.Shin;J.J.Lee
  6. Nukleonika v.44 Application of TSH bioindicator for studying the biological efficiency of radiation A.Cebulska-Wasilewska;K.Rekas;J.K.Kim
  7. Short-Term Bioassay in the Analysis of Complex Environmental Mixtures v.3 A search for the identity of gaseous agents in the ambient air using the Tradescantia bioassay L.A.Schairer;R.C.Sautkulis;N.R.Tempel;M.D.Waters(ed.);S.S.Sandhu(ed.);J.Lewtas(ed.);L.D.Claxton(ed.);S.Nesnow(ed.)
  8. Mut. Res. v.270 Tradescantia stamen-hair system as an excellent botanical tester of mutagenecity: Its responses to ionizing radiations and chemical mutagens, and some synergistic effects found S.Ichikawa
  9. Int. J. Radiat. Biol. v.40 Synergism between EMS and X-rays for the induction of somatic mutations in Tradescantia A.Cebulska-Wasilewska;H.P.Leenhouts;K.H.Chadwick
  10. Jpn. J. Genet. v.68 Synergistic effects of methyl methanesyl-fonate and X-rays in inducing somatic mutations in the stamen hairs of Tradescantia clones, KU 27 and BNL 4430 S.Ichikawa;A.Yamaguchi;M.Okumura
  11. J. Kor. Nuc. Soc. v.13 Mutation frequency of Tradescantia(BNL clone 4430) stamen hairs exposed to low dose of gamma ray in KAERI gamma-field S.H.Kwon;Y.I.Lee;K.H.Chung;J.H.Oh
  12. Environ. Health Perspect. v.37 In situ monitoring with Tradescantia around nuclear power plants S.Ichikawa
  13. Kor. J. Environ. Biol. v.16 Monitoring of environmental radiation using Tradescantia bioindicator J.K.Kim;W.R.Kim;Y.K.Lee;J.S.Kim;H.S.Song;C.Y.Jeong;K.N.Kim;K.P.Hong
  14. Mut. Res. v.270 Tradescantia stamen-hair mutation bioassay on the mutagenicity of radioisotope-contaminated air following the Chernobyl nuclear accident and one year later A.Cebulska-Wasilewska
  15. J. Kor. Assoc. Radiat. Prot. v.23 Neutron dose response of Tradescantia stamen hair pink mutations and RBE J.K.Kim;W.R.Kim
  16. Nukleonika v.40 RBE of 5.6 MeV neutrons assessed for the various biological endpoints in two clones of Tradescantia A.Cebulska-Wasilewska;M.Litwiniszyn;B.Palka
  17. Radioactivity : Risk and Hope, Book of abstract, Workshop in memory of Maria Sklodowska-Curie Biological efficiency of epithermal neutrons estimated from gene mutations in TSH assay K.Rekas;A.Cebulska-Wasilewska;J.Capala
  18. Proc. KARP Autumn Mtg. Application of Tradescantia for studying the effect of boron neutron capture Kim J.K.;Cebulska-Wasilewska,A.
  19. Int. J. Radiat. Biol. v.41 The influence of time between cutting and irradiation on the sensitivity of Tradescantia stamen hairs to mutation induction A.Cebulska-Wasilewska;M.Sijsma;K.H.Chadwick;H.P.Leenhouts
  20. Proc. Florida State Horticultural Society v.77 A simple liquid-culture method of growing plants A.Conger
  21. Korean J. Environ. Agr. v.18 Effect of NaCl treatment and gamma ray irradiation on the induction of pink mutations in hairs of Tradescantia stamen J.K.Kim;W.R.Kim;J.S.Kim;K.N.Kim;K.P.Hong