Effects of Serum on Nitric Oxide Production in Embryonic Mouse Liver Cell Line BNL CL.2

혈청이 마우스 간 세포주 BNL CL.2의 Nitric Oxide 생성에 미치는 영향

  • 김유현 (원광보건대학 임상병리과) ;
  • 김신무 (원광보건대학 임상병리과) ;
  • 배현옥 (원광대학교 의과대학 미생물학 및 면역학교실) ;
  • 유지창 (원광대학교 의과대학 미생물학 및 면역학교실) ;
  • 정헌택 (원광대학교 의과대학 미생물학 및 면역학교실) ;
  • 진효상 (전주대학교 이공대학 생명과학부)
  • Published : 1999.06.01


Nitric oxide (NO) plays an important role in immunologic defense, and influences upon the functioning of secretory tissues and cells. It also exhibits cytotoxic/cytostatic activity as one of major operating effectors of the cellular immunity system. We investigated the effects of serum on the cell damages and NO production in the mouse liver cell line BNL CL.2 to establish the role of NO. We observed that, when BNL CL.2 cells were cultured in serum-free medium, they were induced to cell damage by the stimulation of IFN-$\gamma$ alone or IFN-$\gamma$ plus LPS. Serum-starved cells showed large amount of nitrite accumulation and NO synthase (NOS) expression in response to IFN-$\gamma$ alone in dose- and time- dependent manners, but serum-supplied cells did not The production of NO was blocked by protein tyrosine kinase (PTK) inhibitors, genistein and herbimycin. These results suggest that the deprivation of serum in the BNL CL.2 cell culture medium might primed with the cells to produce NO when the cells are triggered by IFN-$\gamma$ and the involvement of PTK signal transduction pathway in the expression of NOS gene in murine hepatocytes.