Antimutagenic Effect of Genistein toward Environmental Mutagen

환경성 변이원에 대한 Genistein의 항돌연변이성

  • 윤희선 (부산대학교 생물학과) ;
  • 유미애 (부산대학교 분자생물학과) ;
  • 박건영 (부산대학교 식품영양학과) ;
  • 이원호 (부산대학교 생물학과)
  • Published : 1999.10.01

Abstract

This study was carried out to determine the antimutagenic effects of genistein on the somatic mutagenicity induced by aflatoxin B1 (${AFB}_1$), using Drosophila wing spot test system. Mutagen alone or mutagen with genistein were administered to the heterozygous(mwh/+) third instar larvae by feeding, and somatic cell mutations were detected in adult fly wing hairs. Genistein did not show any mutagenicity with the feeding concentrations of 5~15% in the test system. As the feeding concentrations of genistein increased, genistein inhibited the mutagenicity induced by AFB1 (14.6%~62.2% inhibition rate), while as the concentrations of AFB1 increased, small much spots that arise mostly from chromosome deletion and nondisjunction were more strongly suppressed by genistein than the large mwh spots from chromosomal recombination. In each group of different AFB1 concentrations, the rate of inhibition for total mwh spots was dependent on the dose of genistein. These results indicate that genistein have inhibitory effect on the mutagenicity induced by a mtagen, ${AFB}_1$. It seems to suggest that genistein may exert inhibitory effects to mutagenic and/or carcinogenic properties of DNA damaging agents.

Keywords

genistein;AFB1;Drosophila wing spot test system

References

  1. Japan. J. Toxicol. Environ. Health v.40 no.6 Bio-antimutagen detection method with wing spot test by Drosophila melanogaster Sato, T.;H. Nishino;H. Nagase;M. Niikawa
  2. J. Nutr. v.125 Soy and experimental cancer : Animal studies Hawrylewicz, E.J.;J.Z. Jose;H.B. William
  3. Scientific American v.275 no.3 New molecular targets for cancer therapy Oliff, A.;J.B. Gibbs;F. McCormick
  4. J. Nutr. v.125 Soybean phytoestrogen intake and cancer risk Herman, C.;T. Adlercreutz;B.R. Goldin;S.L. Gorbach;K.A.V. Hockerstedt;S. Watanabe;E.K. Hanalainen;M.H. Markkanen;T.H. Makela;K.T. Wahala;T.A. Hase;T. Fotsis
  5. Japan. J. Cancer Res. v.76 Mutagenic potency of heterocyclic amines in the Drosophila wing spot test and its correlation to carcinogenic potency Yoo, M.A.;H. Ryo;T. Todo;S. Kondo
  6. Carcinogenesis v.16 no.11 Genistein suppresses mammary cancer in rats Lamartiniere, C.A.;J.B. Moore;N.M. Brown;R. Thompson;M.J. Hardin;S. Barnes
  7. Proc. Soc. Exp. Biol. Med. v.208 Neonatal genistein chemoprevents mammary cancer Lamartiniere, C.A.;J.B. Moore;M. Holland;S. Barnes
  8. Carcinogenesis v.14 no.9 Cancer chemoprevention :Principle & prospects Morse, M.A.;G.D. Stoner
  9. Chemical Mutagens v.10 In vivo mutagenicity tests using somatic cells of Drosophila melanogaster Wurgler, F.E.;E.W. Vogel;F.J. de Serres(ed.)
  10. J. Nutr. v.125 Genistein, a dietary-derived ingested isoflavonoid, inhibits cell proliferation and in vitro angiogenesis Fotsis, T.;M. Pepper;H. Adlercreutz;T. Hase;R. Montesano;L. Svhweigerer
  11. J. Agric. Food Chem. v.41 Genistein, Daidzein and their β-glycoside conjugates, antitumor isoflavones in soybean foods from American and Asian diets Coward, L.;N.C. Barnes;D.R.K. Setchell;S. Barnes
  12. Environ. Mutag. Carcin. v.14 no.2 Antimutagenic effect of Doenjang-Inhibitory effect of Doenjang stew and soup on the mutagenicity induced by Aflatoxin B₁ Park, K.Y.;S.H. Moon;S.H. Rhee
  13. Biostatistical analysis Multisample hypotheses : The analysis of variance Jerrold, H.Z.
  14. Oncogene v.6 Inhibition of PDGF-induced c-jun and c-fos expression by a tyrosine protein kinase inhibitor Zwiller, J.;P. Sassone-Corsi;K. Kakazu;A.L. Boynton
  15. Cancer Res. v.53 Genistein arrests cell cycle progression at G₂-M Matsukawa, Y.;N. Marui;T. Sakai;Y. Satomi;M. Yoshida;K. Matsumoto;H. Nishino;A. Aoike
  16. Carcinogenesis v.17 no.9 The role of metabolism in mammary epithelial cell growth inhibition by the isoflavones genistein and biochanin A Peterson, T.G.;L. Coward;M. Kirk;C.N. Falany;S. Barnes
  17. Cancer Res. v.53 Antiproliferative effects of isoflavones on human cancer cell lines established from the gastrointestinal tract Yanagihara, K.;A. Ito;T. Toge;M. Numoto
  18. Environ. Mutagen v.6 Somatic mutation and recombination test in Drosophila melanogaster Graf, U.;F.E. Wurgler;A.J. Katz;H.J. Frei;C.B. Hall;P.G. Kale
  19. J. Korean Cancer Assoc. v.30 no.4 Genistein induces inhibition of cell proliferation and programmed cell death in the human cell lines Choi, Y.H.;S.J. Lee;M. Kim;L. Zhang;W.H. Lee;K.Y. Park
  20. Cancer Res. v.49 Inhibitor effects of the tyrosine inhibitor genistein on mammalian DNA topoisomerase II Markovits, J.;C. Linassier;P. Fosse;J. Couprie;J. Pierre;A. Jacquemin -Sablon;J.M. Saucier;J.B. Le Pecq;A.K. Larsen
  21. Nutr. Cancer v.27 no.1 Estrogenic and antiproliferative properties of genistein and other flavonoids in human breast cancer cells in vitro Zava, D.T.;G. Duwe
  22. Experientia v.51 The relationship between age of larvae at mutagen treatment and frequency and size of spots in the somatic mutation and recombination test in Drosophila melanogaster Graf, U.
  23. Can. J. Physiol. Parmacol v.74 Molecular biomarkers for aflatoxins, from adducts to gene mutations to human liver cancer Groopman, J.D.;J.S. Wang;P. Scholl
  24. Mutation Res. v.203 Statistical methods to whether mutagenicity test data from Drosophila assays indicate a positive, negative, or inconclusive result Frei, H.;F.E. Wurgler
  25. Anticancer Research v.14 Antiproliferative effect of genistein and adriamycin against estrogendependent and -independent human breast carcinoma cell lines Monti, E.;B.K. Sinha
  26. J. Korean Soc. Food Sci. Nutr. v.26 no.3 Composition and antioxidative characteristics of phenolic fractions isolated from soybean fermented food Lee, J.S.;H.S. Cheigh
  27. J. Nutr. v.125 The evidence for soybean products as cancer preventive agents Kennedy, A.R.
  28. Scientific American v.275 no.3 Chemoprevention of cancer Greenward, P.
  29. J. Korean Soc. Food Nutr. v.13 no.1 Aflatoxin : factors affecting aflatoxin production Park, K.Y.
  30. Nutr. Cancer v.21 Soy intake and cancer risk : A review of the in vitro and in vivo data Messina, M.;V. Persky;D.R.K. Serchell;S. Barnes