Structure of Spodoptera exigua Nucleopolyhedrovirus p10 Gene

파밤나방 핵다각체병 바이러스의 p10 유전자 구조

  • 최재영 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 우수동 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 홍혜경 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 이해광 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 제연호 (서울대학교 농업생명과학대학 응용생물화학부) ;
  • 강석권 (서울대학교 농업생명과학대학 응용생물화학부)
  • Published : 1999.08.01

Abstract

To develop the baculovirus expression vector system (BEVS) adopting p10 gene promoter of Spodoptera exigua nucleopolyhedrovirus (SeNPV), we characterized the p10 gene of SeNPV. The nucleotide sequence of 545 bases including the coding region of p10 gene was determined. Compared with the previously reported SeNPV p10 gene (Zuidema et al., 1993), 4 bases were different in the 5' and 3' flanking region but no difference was found in the coding region. The p10 gene was located within Xho I 1.5 Kb, Sph 1 2.4 Kb and Cla I 4.0 Kb fragments by Southern hybridization analysis. Also, the Sph I 2.4 Kb and the Cla I 4.0 Kb fragments were cloned and their restriction enzyme maps were determined.