Induction of Lactococcal /beta-Galactosidase in E. coli

E. coli에서 탄수화물원에 따른 Lactococcal /beta-galactosidase의 발현

  • 류현주 (조선대학교 식품영양학과) ;
  • 장지윤 (조선대학교 식품영양학과) ;
  • 이형주 (서울대학교 식품공학과) ;
  • 김정환 (경상대학교 식품공학과) ;
  • 정대균 (경희대학교 유전공학과 및 유전공학연구소) ;
  • 이종훈 (경기대학교 식품생물공학과) ;
  • 장해춘 (조선대학교 식품영양학과)
  • Published : 1999.06.01

Abstract

The structural $\beta$-galactosidase gene (lacZ) from Lactococcus lactis ssp. lactis 7962 was cloned into plamid vector pKF18, which was designated as pKF-gal. Expression of the lacZ from L. lactis 7962 was found to be higher when cells were grown at 3$0^{\circ}C$ than 37$^{\circ}C$. Maximum $\beta$-galactosidase activity was obtained when E. coli/pKF-gal was cultivated for 6hr at 3$0^{\circ}C$ and for 3hr at 37$^{\circ}C$, and L. lactis 7962 was grown for 8hr at 3$0^{\circ}C$. Enzyme induction was achieved by the addition of lactose, galactose, or lactose+IPTG to growing culture. The addition of glucose had no effect on enzyme induction.

Keywords

induction;/beta-galactosidase;L. lactis ssp. lactis 7962;E coli

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