Induction of Lactococcal /beta-Galactosidase in E. coli

E. coli에서 탄수화물원에 따른 Lactococcal /beta-galactosidase의 발현

  • 류현주 (조선대학교 식품영양학과) ;
  • 장지윤 (조선대학교 식품영양학과) ;
  • 이형주 (서울대학교 식품공학과) ;
  • 김정환 (경상대학교 식품공학과) ;
  • 정대균 (경희대학교 유전공학과 및 유전공학연구소) ;
  • 이종훈 (경기대학교 식품생물공학과) ;
  • 장해춘 (조선대학교 식품영양학과)
  • Published : 1999.06.01


The structural $\beta$-galactosidase gene (lacZ) from Lactococcus lactis ssp. lactis 7962 was cloned into plamid vector pKF18, which was designated as pKF-gal. Expression of the lacZ from L. lactis 7962 was found to be higher when cells were grown at 3$0^{\circ}C$ than 37$^{\circ}C$. Maximum $\beta$-galactosidase activity was obtained when E. coli/pKF-gal was cultivated for 6hr at 3$0^{\circ}C$ and for 3hr at 37$^{\circ}C$, and L. lactis 7962 was grown for 8hr at 3$0^{\circ}C$. Enzyme induction was achieved by the addition of lactose, galactose, or lactose+IPTG to growing culture. The addition of glucose had no effect on enzyme induction.


induction;/beta-galactosidase;L. lactis ssp. lactis 7962;E coli


  1. Gene v.167 Plasmids with a kamamycin-resistance gene for site directed mutagenesis using the oilgodeoxyribonucleotide-directed dual amber method Hashimoto-Gotoh, T.;K. Yasojima;A. Tsujimura
  2. J. Bacteriol. v.96 Genetic and enzymatic experiments relating to the tcrtiary structure of β-galactosidase Langridge, J.
  3. Appl. Environ. Microbiol. v.29 Improved medium for lactic Streptococci and their bacteriophage Terzaghi, B.E.;W.E. Sandine
  4. Arch. Microbiol. v.151 Purification and characterization of an inducible β-gal-actosidase from Corynebacterium murisepticum Priyoldar, M.;C.K.K. Nair;D.S. Pradhan
  5. Appl. Environ. Microbiol. v.50 Galactose transport in Streptococcus thermophilus Hutkins, R.;H.A. Morris;L.L. Mckay
  6. J. Mol. Biol. v.69 Lac repressor-operator interaction. Ⅵ. The natural inducer of the lac operon Jobe, A.;S. Bourgeois
  7. Gene v.62 Isolation and structural analysis of the phospho-β-galactosidase from Streptococcus lactis Z268 Boizet, B.;D. Villeval;P. Glos;M. Novel;G. Novel;A. Mercenier
  8. Microbiol. Rev. v.44 Carbohydrate transport in bacteria Dills, S.S.;A. Apperson;M.R. Schmidt;M.H. Saier
  9. Biochem. Biophys. Acta v.23 Properties and induction of β-galactosidase in Bacillus megaterium Landman, O.
  10. Molecular Cloning-A Laboratory Manual(2nd ed.) Sambrook, J.;E. Fritsch;J. Maniatis
  11. J. Bacteriol. v.89 β-Galactosidase of Streptococcus lactis Gui, J.E.;W.E. Sandine;P.R. Elliker
  12. Gene v.106 Cloning and analysis of the β-galactosidase-encoding gene from Clostridium thermosulfurogens EM1 Burchhardt, G.;H. Bahl
  13. Cold Spring Harbor Symp. Quant. Biol. v.26 On the regulation of gene activity Jocob, F.;F. Monod
  14. Gene v.33 Improved M13 phage cloning vectors and host strains:Nucleotide sequences of the M13mp18 and pUC19 vectors Yanisch-Perron, C.;J. Vierira;J. Messing
  15. Gene v.62 Nucleotide of the β-D-galactosidase galactohydrolase gene of Lactobacillus casei;Comparison to analogous pbg genes of other Gram positive organisms Porter, E. V.;B.M. Chassy
  16. J. Bacteriol. v.169 Regulation of β-galactosidase transport and accumulation in heterofermentative lactic acid vacteria Romano, A.H.;G. Brino;A. Peterkofsky;J. Reizer
  17. J. Bacteriol. v.142 Physiological studies of β-galactosidase induction in Kluyveromyces lactis Dickerson, R.C.;J.S. Markin
  18. Appl. Environ. Microbiol. v.51 Transport and metabolism of lactose, glucose, and galactose in homofermentative Lactobacilli Hickey, M.W.;A.J. Hillier;G.R. Jago
  19. J. Dairy Sci. v.45 Different pathways of lactose metabolism of Streptococcus lactis and their sensitivity to antiboiotics Vakil, J.R.;K.M. Shahani
  20. J. Bacteriol. v.180 Transcriptional regulation and evolution of lactose genes in the galactose-lactose operon of Lactococcus lactis NCDO 2054 Vaughan, E.E.;R.D. Pridmore;B. Mollet
  21. The Operon lactose permease protein and thiogalactoside transacetylase J. Miller(ed.);W.S. Reznikoff(ed.)
  22. J. Bacteriol. v.80 β-Gal-actosidase of Paracolobactrum aerogenoides Anderson, J.M.;H.V. Rickenberg
  23. The Lactose Operson Glucose effects:Inducer exclusion and repression Magasanik, B.;J.R. Beckwith(ed.);D. Zipser(ed.)
  24. J. Gen. Physiol. v.36 The mechanism of the synthesis of enzymes. Ⅰ. Development of a system suitable for studying this phenomenon Koppel, J.L.;C.J. Porter;B.F. Croker
  25. Experiment in Molecular Genetics Miller, J.H.
  26. Nature v.197 β-Galactosidase of Shigella sonnei Clausen, C.R.;M. Nakanamura
  27. J. Bacteriol. v.80 Occurence of β-galactosidase in the genus Shigella Rickenberg, H.V.
  28. J. Bacteriol v.89 β-Galactosidase of Streptococcus lactis Citti, J.E.;W.E. Sandine;P.R. Elliker
  29. Biotechnol. Letters v.29 Cloning and nucleotide sequence of the β-galactosidase gene from Lactococcus lactis ssp. lactis ATCC 7962 Lee, J.M.;D.K. Chung;J.H.Park;W.K.Lee;H.C.Chang;J.H. Kim;H.J. Lee
  30. J. Microbiol. Biotechnol. v.6 Molecular cloning of β-D-galactosidase gene from Lactococcus lactis subsp. lactis 7962 Chang, H.C.;Y.D. Choi;H.J. Lee
  31. Seoul National University Characterization of β-galactosidase gene from L. lactis ssp. lactis 7962 Lee, J.M.
  32. J. Bacteriol. v.86 Induction of lactose utilization in Streptococcus aureus McClatchy, J.K.;E.D. Rosenblum