Purification and Characterization of Aminoglycoside-Resistant inhibitior from methylotrophic Actinomycetes

Methanol 자화 방선균으로부터 Aminoglycoside 내성 저해물질의 정제 및 특성

  • 김현수 (계명대학교 자연과학대학 미생물학과) ;
  • 신재욱 (일양약품 중앙연구소)
  • Published : 1999.06.01

Abstract

Methylotrophic actinomycetes No. 155 produced an aminoglycoside antibiotics(AG)-resistant inhibitor. We have previously reported that the inhibitor shows strong inhibition to sisomicin-resistant strain. In order to understand the functions of inhibitor and sisomicin-resistance, characterizations and purification of inhibitor were investigated. Strain No. 155 was tentatively identified as Nocardiopsis sp. based on morphological and some physiological characteristics. In the antimicrobial activity test, the addition of inhibitor to sisomicin showed a reduction effect of MIC on the test strains such as Gram(+), Gram(-) bacteria and yeasts. The combination of the inhibitor and various antibiotics revealed synergistic against E. coli K-12 and B. subtilis PCI 219. The induced intracellular proteins from sisomicin-resistant strain exhibited the sisomicin inactivation by invitro test. And the induced intracellular proteins were inactivated by addition of the inhibitor. The inhibitor compound was purified by anion exchange chromatography(Dowex-1) and HPLC using Asahipak ES-502C column. The purified inhibitor compound was detected in a single peak(above 98.5% purity) through the HPLC analysis.

Keywords

aminoglycoside antibiotics;sisomicin;methylotrophic actinomyceted;sisomicin-resistant inhibitor

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