Immobilization of Agarase for the Agarooligosaccharide Production

한천올리고당의 생산을 위한 한천분해효소의 고정화

  • 임동중 (부경대학교 생물공학과) ;
  • 김봉조 (부경대학교 생물공학과) ;
  • 배승권 (부경대학교 생물공학과) ;
  • 김종덕 (여수대학교 생물공학과) ;
  • 공재열 (부경대학교 생물공학과)
  • Published : 1999.06.01

Abstract

The condition for immobilization of the partially purified agarase from Bacillus cereus ASK202 and the properties of the immobilized enzyme have been investigated. Agarase was immobilized on various supports by entrapment method. The enzyme immobilized on Na-alginate bead showed the highest activity among those studied. The optimal reaction conditions of the immobilized agarase were obtained in 3%(w/v) Na-alginate for the matrix, bead diameter of 2.5mm, 1 unit of agarase solution and 1.0%(w/v) calcium chloride solution. The optimum pH and temperature of the immobilized agarase were pH and temperature of the immobilized agarase were pH 7.0 and 4$0^{\circ}C$, respectively. Km and Vmax values were 0.5mg/ml.min, respectively. The immobilized agarase conerted agar to agarobiose, and their total conversion ratio under the optimal condition was 89%.

Keywords

agarase;agarooligosaccharides;immobilization;gel bead;Na-alginate

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