Properties of an Extracellular Amylase Produced by the Marine Halophilic Bacterium Vibrio alginolyticus

해양 호염성 세균 Vibrio alginolyticus가 생산하는 Extracellular Amylase의 특성

  • 김영재 (창원대학교 자연과학대학 미생물학과)
  • Published : 1999.06.01


V. alginolyticus 138-2, a marine halophilic bacterium, produced an extracellular amylase with a molecular weight of ca. 56,000. The analysis of the digestion products of soluble starch by thin layer chromatography(TLC) revealed that the extracellular amylase of V. alginolyticus 138-2 is a saccharifying-type alpha-amylase. The alpha-amylase activity of the culture supernatant of soluble starch was optimal at pH 6.0 and 45$^{\circ}C$. Ca2+ slightly increased the alpha-amylase activity, whereas Hg2+, An2+, Cu2+, Ni2+, Fe2+, and Mn2+inhibited the enzymatic activity. Alkylating thiol group agent, iodoacetic acid did not affect the alpha-amylase activity, but reduced thiol reagents such as dithiothreitol, cysteine, and beta-mercaptoethanol stimulated theenzymatic activity. On the other hand, even if V. alginolyticus 138-2 is a marine halophilic bacterium, its alpha-amylase activity was significantly inhibited by NaCl.


  1. Appl. Environ. Microbiol. v.60 Properties and active center of the thermostable branching enzyme from Bacillus stearothermophilus Takata, H.;T. Takaha;T. Kuriki;S. Okada;M. Takagi;T. Imanaka
  2. Appl. Environ. Microbiol. v.59 Cloning of the beta-amylase gene from Bacillus cereus and characteristics if the primary structure of the enzyme Nanmori, T.;M. Nagai;Y. Shimizu;R. Shinke;B. Mikami
  3. Biotechnol. Appl. Biochem. v.12 Characterization of a thermostable Bacillus stearothermophilus alpha-amylase Vihinen, M.;P. Mantsala
  4. Biosci. Biotech. Biochem. v.61 Purification and some properties of an alpha-amylase from an anaerobic bacterium isolated from coastal sediment Sugita, H.;A. Kuruma;Y. Deguchi.
  5. Nature (London) v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli, U. K.
  6. Biochem. Soc. Trans. v.24 The alpha-amylase of Bacillus coagulans Keating, L.A.;C.T. Kelly;W.M. Fogarty
  7. Enzyme Microb. Technol. v.9 Purification and chemical characterization of thermostable amylase produced by Bacillus stearothermophilus Srivastava, R.A.K.
  8. Arr. Biol. Chem. v.53 Novel maltose-producing amylase from Bacillus megaterium G-2 Takasaki, Y.
  9. J. Appl. Chem. Biotechnol. v.25 Purification and properties of beta-amylase produced by Bacillus polymyxa Fogarty, W.M.;P.J. Griffin
  10. J. Biochem. v.109 Fluorescence quenching studies on the characterization of energy generated at the NADH:quinone oxidoreductase and quinol oxidase segments of marine bacteria Kim, Y.J.;S. Mizushima;H. Tokuda
  11. Agr. Biol. Chem. v.38 Studies on betaamylase of Bacillus megaterium strain No. 32 Higashihara, M.;S. Okada
  12. Biotechnol. Appl. Biochem. v.8 Purification of glucoamylase by acarbose (BAY g-5421) affinity chromatography Ono, K.;E.E. Smith
  13. Carbohydr. Res. v.309 Mechanism of action and the substrate-dependent pH maximum shift of the alpha-amylase of Bacillus coagulans Keating, L.;C. Kelly;W. Fogarty
  14. Ital. J. Biochem. v.32 Purification and properties of a glucoamylase fraction from the culture filtrate of Rhizopus modosis Muthukumaran, N.;S.C. Dhar
  15. J. Biol. Chem. v.244 The extracellular alpha-amylase of Bacillus stearothermophilus Pfueller, S.L.;W.H. Elliott
  16. World J. Microbiol. Biotechnol. v.10 Purification properties of bata-amylase from Bacillus circulans S 31 Kwan, H.S.;K.H. So.;K.Y. Chan;S.C. Cheng
  17. J. Biochem. (Tokyo) v.84 Purification and some properties of three forms of glucoamylase from a Rhizopus species Takahashi, T.;Y. Tsuchida;M. Irie
  18. Arch. Biochem. Biophys. v.155 A thermophilic extracellular alpha-amylase from Bacillus licheniformis Saito, N.
  19. Gene v.19 Molecular cloning of alpha-amylase gene from Bacillus amyloliquefaciens and its expression in B. subtilis Palva, I.
  20. Biotechnol. Bioeng. v.33 High-temperature allkaline alpha-amylase from Bacillus licheniformis TCRDC-B13 Bajpai, P.;P.K. Bajpai
  21. J. Biol. Chem. v.259 $Na^+$ is translocated at the NADH:quinone oxidoreductase segment in the respiratory chain of Vibrio alginolyticus Tokuda, H.;T. Unemoto
  22. J. Bacteriol. v.165 Cloning and characterization of the beta-amylase genefrom Bacillus polymyxa Friedberg, F.;C. Rhodes
  23. J. Biol. Chem. v.258 Amino acid sequence of alpha-amylase from Bacillus amyloliquefaciens deduced from the nucleotide sequence of the cloned gene Takkinen, K.;R. F. Petterson;N. Kalkkinen;I. Palva;H. Soderlund;L. Kaariainen
  24. Folia Micrbiol v.39 Beta-amylase from Bacillus megaterium Ray. R.R.;S.C. Jana;G. Nada
  25. Handbook of Amylases and Related Enzymes Bacterial alpha-amylase (liquefying and saccharifying types) of Bacillus subtilis and related bacteria Yamamoto, T.;The Amylase Research Society of Japan(ed.)