Antimutagenic Effect of Tansen (Salvia miltriorrhiza Bunge)

단삼의 항산화적 항돌연변이 효과

  • 안병용 ;
  • 김동길 (우석대학교 생물산업생명공학부) ;
  • 최동성 (원광대학교 생명자원과학부)
  • Published : 1999.06.01


To confirm the effects of binlang(Areca catechu L.) and tansen(Salvia miltriorrhiza bung) on the mutagenicity induced by hydrogen peroxide, SOS Chromotest with Escherichia coli PQ37 and Ames test with Salmonella typhimurium TA104 were perlormed. Methanol-soluble parts of their water extracts showed high inhibitory effect against the mutagenicity of hydrogen peroxide in two bacterial mutation assays. Step-wise fractionation of methanol-soluble part from tansen was done using ethyl acetate, butanol and water. Among these fraction was further partitioned by Sephadex LH-20 column chromtography, and 6 subfractions were obtained. The fraction III showed the strongest inhibitory effects against the mutagenic activities induced by hydrogen peroxide. The inhibition rates of fraction III at concentration of 500$\mu\textrm{g}$/assay were 28%, 30% and 15% against 4-NQO, MNNG and B(a)P, respectively. But the mutagenic potency of AFB1 was increased.


SOS Chromotest;Ames test;antimutagenic effect;Salvia miltriorrhiza Bunge


  1. Mutation Res. v.336 The effects of hydrogen peroxide on DNA repair activities Hu, J.J.;N. Dubin;D. Kurland;B.L. Ma;G.C. Roush
  2. Cancer Res. v.48 Cytotoxic and genotoxic effects of Areca-related compounds in cultures human buccal epithelial cells Kristina, S.;L. Yun;N. Jagadeesan;B. Helmut;A. Kristina;C. G. Roland
  3. Mutation Res. v.321 Inhibition of the bacterial mutagenicity of MNNG by ascorbic acid and ascobyl palmitate Tyrsina, E.G.;O.G. Rossikhina;S.K. Abilev;Y.A. Tyrsin
  4. Int. J. Cancer. v.47 Cytotoxic and cytostatic of arecoline and sodium nitrite on human cell in vitro Wary, K.K.;R.N. Sbaran
  5. J. Nat. Prod. v.51 Plant antimutagenic agents, 3. Coumarins Wall, M.E.;M.C. Wani;G. Manikumar;T.J. Hughes;H. Taylor;R. Mcgivney;J. Waner
  6. Korean J. Food Sci. Technol. v.30 Bio-antimutagenic effects of water extract from Rehmannia glutinosa Liboschitz in SOS Chromotest Ahn, B.Y.;K.S. Lee;G.S. Song;I.K. Maeng;D.S. Choi
  7. FEBS Lett. v.281 DNA damage by oxygen-derived species. Its mechanism and measurement in mamalian systems Halliwell, B.;O.I. Aruoma
  8. Mutation Res. v.266 Effects of various chemical compounds on spontaneous and hydrogen peroxide induced reversion in Salmonella typhimurium TA104 Han, J.S.
  9. Korean J. Food Sci. Technol. v.29 Effect of hot water extracts from medicinal plants on the mutagenicity of indirect mutagens Song, G.S.;B.Y. Ahn;K.S. Lee;I.K. Maeng;D.S. Choi
  10. Cancer Detect. Prev. v.14 Oxidative stress, DNA repair and cancer susceptibility Pero, R.W.;G.C. Roush;M.M. Markowitz;D.G. Miller
  11. Mutation Res. v.87 Mechanisms of inhibition of free radical processes in mutagenesis and carcinogenesis Simic, M.G.
  12. Mutation Res. v.113 Revised methods for the Salmonella mutagenicity test Maron, D.M.;B.N. Ames
  13. Journal of HRDC v.3 The inhibitory effects of medicinal plants on the mutagenicity of hydrogen peroxide Ahn, B.Y.;I.K. Maeng;D.S. Choi
  14. J. Environ. Pathol. Toxicol. v.4 Differential effects of cytochrome P450-inducers on promutagen activation capabilities and enzymatic activities of S-9 from rat liver Ong, T.M.;M. Mukhtar;C.R. Wolf;E. Zeiger
  15. Mutation Res. v.147 The SOS Chromotest, a colorimetric bacterial assay for genotoxins: Procedures Quillardet, P.;M. Hofnung
  16. Mutation Res. v.265 Modulating effect of tanshinones on mutahenic activity of Trp-P-1 and benzo(a)pyrene in Salmonella typhimurium Sato, M.;T. Sato;Y. Ose;H. Nagase;H. Kito;Y. Saki
  17. Mutation Res. v.187 Effects of antimutagen cinnamaldehyde on reversion and survival of selected Salmonella tester strains Silva, H.V.;D.M. Shankel
  18. J. Agric. Food Chem. v.38 Antioxidative components of Tanshen (Saliva miltiorrhiza Bunge) Zahang, K.;Y. Bao;P. Wu;R. T. Rosen;C. Ho
  19. Food Biotechnol v.6 Antimutagenicity screening of water extracts from Chinese herb with SOS Chromotest with several derect mutagens Song, G.S.;B.Y. Ahn;I.K. Maeng;Y.J. Kwon;S.B. Han;D.S. Choi
  20. Mutation Res. v.202 Dietary inhibitors of mutagenesis and carcinogenesis Hayatsu, H.;S. Arimoto;T. Negishi.