Screening and Taxonomic Charactrization of D-Amino Acid Aminotransferase-producing Thermophiles

D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

  • 곽미선 (생명공학연구소 미생물전환연구UNIT, 충남대학교 제약학과) ;
  • 이승구 (생명공학연구소 미생물전환연구UNIT) ;
  • 정상철 (제일제당 종합연구소) ;
  • 서승현 (제일제당 종합연구소) ;
  • 이재흥 (제일제당 종합연구소) ;
  • 전영중 (제일제당 종합연구소) ;
  • 김영호 (충남대학교 제약학과) ;
  • 성문희 (생명공학연구소 미생물전환연구UNIT)
  • Published : 1999.06.01

Abstract

To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two thermophiles, Bacillus sp. Lk-1 and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-01, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

Keywords

screening;D-amino acid aminotransferase;taxonomic characterization;thermophiles

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