Screening and Taxonomic Charactrization of D-Amino Acid Aminotransferase-producing Thermophiles

D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

  • 곽미선 (생명공학연구소 미생물전환연구UNIT, 충남대학교 제약학과) ;
  • 이승구 (생명공학연구소 미생물전환연구UNIT) ;
  • 정상철 (제일제당 종합연구소) ;
  • 서승현 (제일제당 종합연구소) ;
  • 이재흥 (제일제당 종합연구소) ;
  • 전영중 (제일제당 종합연구소) ;
  • 김영호 (충남대학교 제약학과) ;
  • 성문희 (생명공학연구소 미생물전환연구UNIT)
  • Published : 1999.06.01


To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two thermophiles, Bacillus sp. Lk-1 and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-01, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.


screening;D-amino acid aminotransferase;taxonomic characterization;thermophiles


  1. J. Biotechnol. v.8 Enantioselective of various D-amino acids by a multi-enzyme system Nakajima, N.;K. Tanizawa;H. Tanaka;K. Soda
  2. Bergey's Manual of Systematic Bacteriology v.2 Endospore-forming Gram-positive rods and cocci Sneath, P.H.A.;P.H.A. Sneath(ed.);N.S. Mair(ed.);M.E. Sharpe(ed.);J.G. Holt(ed.)
  3. Biochem. Biophys. Acta v.1350 Nucleotide sequence of the Bacillus lichieniformis ATCC 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species Taylor, P.P;I.G. Fotheringham
  4. Appl. Environ. Microbiol. v.63 Synthesis of optically active amino acids from alpha-keto acids with Escherichia colicells expressing heterologous genes Galkin, A.;L. Kulakoca;T. Yoshimura;K. Soda;N. Esaki
  5. J. Biol. Chem v.264 The primary structure of thermostable D-amino acid aminotransferase from a thermophilic Bacillus species and its correlation woth L-amino acid aminotransferases Tanizawa, K.;S. Asano,;Y. Masu;S. Kuramitsu;H. Kagamiyama;H. Tanaka;K. Soda
  6. Anal. Biochem. v.27 Spectrometric determination of pyruvic acid by the salicylaldehyde method Berntsson, S.
  7. J. Bacteriol. v.174 Effect of D-amino acids on structure and synthesis of peptidoglycan in Escherichia coli Caparros, M.;A.G. Pisabarro;M.A. Pedro
  8. J. Mol. Catal. B.:Enzymatic v.4 Industrial biotransformations for the production of D-amino acids Yagasaki, M.;A. Ozaki
  9. J. Biol. Chem. v.264 Thermostable D-amino acid aminotransferase from a thermophilic Bacillus species;Putification, characterization, and active site sequence determination Tanizawa, K.;T. Masu;S. Asano;H. Tanaka;K. Soda
  10. Biosci. Biotechnol. Biochem. v.59 Cloning, purification, and properties of a cofactor-independent glutamate racemase from Lactobacillus brevis ATCC8287 Yagasaki, M.;K. Iwata;S. Ishino;M. Azuma;A. Ozaki
  11. J. Ferment. Technol. v.57 Microbial transformation of hydantoins to N-carbamoyl-D-amino acids Takahashi, S.;T. Ohashi;Y. Kii;H. Kumagai;H. Yamada
  12. J. Bacteriol v.177 Staphylococcus haemolyticus contains two D-glutamic acid biosynthetic activities, a glutamate resemase and a D-amino acid transaminase Pucci, M.J.;J.A. Thanassi;H.T. Ho;P.I. Falk;T.I. Dougherty
  13. Hakkokogaku v.61 Microbial synthesis of D-amino acids frin DL-5-substituted hydantoins Takahashi, S.
  14. Thermophillic Bacteria Kristjansson, J. K.;J. K. Kristjansson(ed.)