Inhibitory Activities of Basidiomycetes on Prolyl Endopeptidase, Acetylcholine Esterase and Coagulation

담자균 추출물의 Prolyl Endopeptidase, Acetylcholine Esterase 저해 및 항혈전 응고활성

  • Lee, Hyun-Jin (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Kim, Jong-Sik (Department of Chemistry, Konyang University) ;
  • Heo, Gun-Young (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Lee, Kyung-Bok (College of Medicine, Konyang University) ;
  • Rhee, In-Koo (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University) ;
  • Song, Kyung-Sik (Department of Agricultural Chemistry, College of Agriculture, Kyungpook National University)
  • Published : 1999.11.30

Abstract

Fifty six mycelial cultured Basidiomycetes were screened for their inhibitory effects against prolyl endopeptidase(PEP), acetylcholine esterase(AChE) and thrombus coagulation. Out of them, methanolic extract of mycelium and/or ethylacetate(EtOAc) soluble fraction from culture broth of Peniophora quercina, Amanita aspera, Phellinus chrysoloma, Grifola frondosa, Wolfiporia extensa, Clavicorona pyxidata and Phanerochaete sordida inhibited more than 90% of PEP activity at 40 ppm. The extracts of Lenzites betulina, Phellinus chrysoloma, Wolfiporia extensa, Phanerochaete sorrlida, Hypocrea nigricans, Coriolus azureus, Flammulina velutipes, Phlebiopsis gigantea and Bondarzewia montana exhibited about 40% of inhibitory activity against AChE at 40 ppm. In thrombin times assay, the extracts of Amanita aspera, Oxyporus latemarginata, Peniophora quercina, Fomes fomenfarius, Trametes versicolor, and Phlebiopsis gigantea delayed coagulation of thrombus about two to three times over control at ca 550 ppm. In activated partial thromboplastin times assay, none of the tested Basidiomycetes showed significant effect.