Mechanism of Regulation of the pts Promoter Transcription Initiation by Carbon Sources in Escherichia coli

Carbon Source의 변화에 의한 대장균의 pts Promoter 전사 조절 기작

  • Kim, Soon-Young (Department of Food Science and Technology, Seoul National University) ;
  • Kwon, Hyuk-Ran (Department of Microbiology, College of Medicine, Chungbuk National University) ;
  • Shin, Dong-Woo (Department of Food Science and Technology, Seoul National University) ;
  • Ryu, Sang-Ryeol (Department of Food Science and Technology, Seoul National University)
  • 김순영 (서울대학교 식품공학과) ;
  • 권혁란 (충북대학교 의과대학 미생물학교실) ;
  • 신동우 (서울대학교 식품공학과) ;
  • 유상렬 (서울대학교 식품공학과)
  • Published : 1999.11.30

Abstract

The pts operon, which encodes several factors in the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) of Escherichia coli, has multiple promoters which respond to different signals to facilitate quick adaptation to changes in growth conditions. The influence of an 1 kbp DNA region upstream of the pts P0 promoter on pts expression was studied in vitro by employing the DNA templates containing both P0 and P1 promoter with or without the 1 kbp upstream DNA region for in vitro transcription assay. The 1 kbp DNA region upstream of the pts P0 promoter, however, had no effect on pts transcription in vitro. The intracellular concentration of cAMP was measured when cells were grown in the presence of glucose, mannose, or mannitol. The transcription of P0 was increased maximally in the presence of glucose even though the concentration of cAMP in the condition was lowest while the transcription from the P1b was highest when cells were grown in the presence of mannose or mannitol even though the intracellular concentration of cAMP was lower than cells grown in the absence of the sugar. These results suggest the possibility of the existence of a glucose inducible repressor specific for the P0 promoter and a second repressor that is inducible by glucose, mannose and mannitol specific for the P1 promoter.