Isolation and Structural Determination of Antifungal Antibiotic from Streptomyces hygroscopicus MJM1004

Streptomyces hygroscopicus MJM1004가 생산하는 항진균성 항생 물질의 분리 및 구조 결정

  • Bae, Ju-Yun (Institute of Biological Science and Biotechnology, Myongji University) ;
  • Kwon, Hyong-Jin (Institute of Biological Science and Biotechnology, Myongji University) ;
  • Suh, Joo-Won (Institute of Biological Science and Biotechnology, Myongji University)
  • 배주윤 (명지대학교 생명공학연구소, 생명과학과) ;
  • 권형진 (명지대학교 생명공학연구소, 생명과학과) ;
  • 서주원 (명지대학교 생명공학연구소, 생명과학과)
  • Published : 1999.11.30

Abstract

Several Streptomyces strains were tested for potent antifungal agents active against phytopathogenic fungi. Among the tested, S. hygroscopicus MJM1004 showed a potent antifungal activity when assayed using Candida albicans as indicator organism. With the strain of MJM1004, fermentation medium for the production of an antifungal agent was developed with varying carbon sources, nitrogen sources, and mineral elements, which resulted in the highest productivity in the medium containing 2% soybean meal, 1% glucose, 2% starch, 0.3% $CaCO_3$, 0.05% $MgSO_4{\cdot}7H_2O$, 0.05% $K_2HPO_4$. The active compound showed a broad spectrum of antifungal activity against several plant pathogenic fungi. The antifungal compound was purified and showed the physicochemical characteristics similar to azalomycin F complex in NMR and MS analysis.