Isolation and Structure Determination of Antioxidants from the Root of Paeonia lactiflora

작약(芍藥)(Paeonia lactiflora) 뿌리로부터 항산화활성 물질의 분리

  • Bang, Myun-Ho (Department of Life Science and Institute of Life Science & Resources, Kyunghee University) ;
  • Song, Jung-Choon (National Crop Experiment Station, RDA) ;
  • Lee, Sang-Yang (National Crop Experiment Station, RDA) ;
  • Park, Nam-Kyu (National Crop Experiment Station, RDA) ;
  • Baek, Nam-In (Department of Life Science and Institute of Life Science & Resources, Kyunghee University)
  • 방면호 (경희대학교 생명과학부 및 생명자원과학연구원) ;
  • 송정춘 (농촌진흥청 작물시험장 품질이용과) ;
  • 이상양 (농촌진흥청 작물시험장 품질이용과) ;
  • 박남규 (농촌진흥청 작물시험장 품질이용과) ;
  • 백남인 (경희대학교 생명과학부 및 생명자원과학연구원)
  • Published : 1999.05.31

Abstract

On the purpose of development of novel antioxidative compounds from natural sources, 38 plants expected to show antioxidant activity have been examined concerning DPPH radical scavenging activity. Among them, thirteen plants, including Paeoniae radix, the root of Paeonia lactiflora, exhibited the activity. In order to isolate active component, the root was extracted in 80% aqueous MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. Silica gel column chromatographies of the EtOAc and n-BuOH fraction exhibiting antioxidant activity. were repeatedly carried out with monitoring by DPPH assay to afford three active compounds. On the basis of spectral data and the chemical characteristics, the structures of the compounds were determined as (+)-catechin, $1,2,3,4-tetragalloyl-6-digalloyl-{\beta}-D-glucose$ and $1,2,3,4,6-penta-galloyl-{\beta}-D-glucose$.