Isolation and Identification of Genes Regulated by Iron Using mRNA Differential Display

mRNA differential display를 이용한 철에 의해 조절되는 유전자들의 분리 및 동정

  • Lee, Jung-Lim (Dept. of Food Science and Institute of Genetic Engineering, College of Industry, Kyunghee University) ;
  • Park, Jong-Hwan (Dept. of Food Science and Institute of Genetic Engineering, College of Industry, Kyunghee University) ;
  • Kim, Hae-Yeong (Dept. of Food Science and Institute of Genetic Engineering, College of Industry, Kyunghee University)
  • 이중림 (경희대학교 산업대학 식품가공학과 및 유전공학연구소) ;
  • 박종환 (경희대학교 산업대학 식품가공학과 및 유전공학연구소) ;
  • 김해영 (경희대학교 산업대학 식품가공학과 및 유전공학연구소)
  • Published : 1999.05.31

Abstract

Iron is an essential nutrient but potentially toxic element in human. To identify the effects of iron on the gene expression of mammalian cell, we have isolated several genes that are regulated by iron using the RNA differential display method. RNAs were isolated from HeLa cells treated with iron supplement or iron chelator. A total of 24 genes were isolated and of these, four genes were identified by DNA sequencing and northern blot.