Fermented Extracts of Korean Mistletoe with Lactobacillus (FKM-110) Stimulate Macrophage and Inhibit Tumor Metastasis

유산균으로 발효된 한국산 겨우살이 추출물의 Macrophage 자극에 의한 면역학적 활성화와 종양전이 억제효과

  • Yoon, Taek-Joon (Institute of Biomedical Research center, Han Dong University) ;
  • Yoo, Yung-Choon (Department of Microbiology, Konyang University School of Medicine) ;
  • Kang, Tae-Bong (Institute of Biomedical Research center, Han Dong University) ;
  • Lee, Kwan-Hee (Institute of Biomedical Research center, Han Dong University) ;
  • Kwak, Jin-Hwan (Institute of Biomedical Research center, Han Dong University) ;
  • Baek, Young-Jin (R & D Center, Korea Yakult Co., Ltd) ;
  • Huh, Chul-Sung (R & D Center, Korea Yakult Co., Ltd) ;
  • Kim, Jong-Bae (Institute of Biomedical Research center, Han Dong University, Animal Resources Center, College of Animal Husbandry, Kon-Kuk University)
  • 윤택준 (한동대학교 생의학 연구소) ;
  • 유영춘 (건양 대학교 의과대학 미생물학 교실) ;
  • 강태봉 (한동대학교 생의학 연구소) ;
  • 이관희 (한동대학교 생의학 연구소) ;
  • 곽진환 (한동대학교 생의학 연구소) ;
  • 백영진 (한국 야쿠르트 중앙연구소) ;
  • 허철성 (한국 야쿠르트 중앙연구소) ;
  • 김종배 (한동대학교 생의학 연구소, 건국대학교 동물자원연구센터)
  • Published : 1999.06.30

Abstract

Based on the results that the extract of Korean mistletoe (KM-110) has immunological and anti-tumor activities and its main component is lectin called KML-U, this study was carried out to investigate the immunostimulatory and anti-tumor activities of FKM-110, fermented KM-110 with lactobacillus, as a basic study for the development of functional food with anti-tumor activity. The amount of lectin after fermentation determined by ELISA was varied with the fermentation time and kinds of lactobacillus. Cytotoxic effects of FKM-110 on the various tumor cells was significant and dependent on the concentration of KML-U and the kinds of lactobacillus. FKM-110 stimulated macrophage and resulted in the secretion of some cytokines such as IL-1 and $IFN-{\gamma}$, but this effect was not correlated with the concentration of lectin. FKM-110 fermented with Marshall Lactobacillus casei showed the most potent antitumor activity in experimental and spontaneous metastasis models. When yoghurt produced with KM-110, Marshall Lactobacillus casei and skim milk was administered orally to mouse, the metastasis of tumor cells was significantly inhibited.